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Product Info Summary
| SKU: | M04507 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-SCAP Antibody (N-term)
SKU/Catalog Number
M04507
Size
50 µl
Description
Boster Bio Anti-SCAP Antibody (N-term) (Catalog # M04507). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-SCAP Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04507)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1992C245.37.16
Isotype
IgG1,κ
Immunogen
This SCAP antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 49-83 amino acids from the N-terminal region of human SCAP.
Reactive Species
M04507 is reactive to SCAP in Human
Calculated molecular weight
139.7 kDa
Background of SCAP
Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04507 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
Validation Images & Assay Conditions
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All lanes : Anti-SCAP Antibody (N-term) at 1:4000 dilution
Lane 1: HT-29 whole cell lysate
Lane 2: LNCaP whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 140 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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