Product Info Summary
SKU: | A10412-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-SCEL Antibody Picoband™
SKU/Catalog Number
A10412-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SCEL Antibody Picoband™ catalog # A10412-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SCEL Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10412-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SCEL, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10412-1 is reactive to SCEL in Human, Mouse, Rat
Applications
A10412-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
78 kDa
Calculated molecular weight
77.552kDa
Background of SCEL
Sciellin is a protein that in humans is encoded by the SCEL gene. The protein encoded by this gene is a precursor to the cornified envelope of terminally differentiated keratinocytes. This protein localizes to the periphery of cells and may function in the assembly or regulation of proteins in the cornified envelope. Transcript variants encoding different isoforms exist. A transcript variant utilizing an alternative polyA signal has been described in the literature, but its full-length nature has not been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SCEL using anti-SCEL antibody (A10412-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEP2 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: rat RH35 whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse NTH/3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCEL antigen affinity purified polyclonal antibody (Catalog # A10412-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCEL at approximately 78KD. The expected band size for SCEL is at 78KD.
Protein Target Info & Infographic
Gene/Protein Information For SCEL (Source: Uniprot.org, NCBI)
Gene Name
SCEL
Full Name
Sciellin
Weight
77.552kDa
Alternative Names
FLJ21667; MGC22531; sciellin SCEL sciellin sciellin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SCEL, check out the SCEL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SCEL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SCEL Antibody Picoband™ (A10412-1)
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No publications found for A10412-1
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