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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||IP, IHC, WB|
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Anti-SCGN Monoclonal Antibody
Boster Bio Anti-SCGN Monoclonal Antibody catalog # M10629. Tested in IP, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SCGN Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M10629)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human SCGN Expressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M10629 is reactive to SCGN in Human, Mouse, Rat
M10629 is guaranteed for IP, IHC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
WB 1:500~1:2000 IHC 1:50~1:200 IP 1:50
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SCGN using anti-SCGN antibody (M10629) in human fetal brain lysate.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCGN antigen affinity purified polyclonal antibody (Catalog # M10629) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Gene/Protein Information For SCGN (Source: Uniprot.Org, NCBI)
CALBL; DJ501N12.8; SCGN; SECRET; Secretagogin; secretagogin, EF-hand calcium binding protein; SECRETsecretagogin; SEGN; Setagin SCGN CALBL, DJ501N12.8, SECRET, SEGN, setagin secretagogin, EF-hand calcium binding protein secretagogin*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on SCGN, check out the SCGN Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SCGN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for M10629
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