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- Table of Contents
SDF2L1 antibody (A13464) performs well in ELISA, IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of SDF2L1 using anti-SDF2L1 antibody (A13464).
SDF2L1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SDF2L1 Antibody (A13464) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
SDF2L1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SDF2L1 Antibody (A13464) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-SDF2L1 Antibody Picoband®
SDF2L1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC, WB |
Tested Applications
SDF2L1 antibody was positively detected by WB in:
human K562 whole cell, human 293T whole cell, human HepG2 whole cell, human MCF-7 whole cell, rat pancreas tissue, rat testis tissue, mouse pancreas tissue, mouse testis tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SDF2L1 antibody was positively detected by IHC in:
human liver cancer tissue, human lung cancer tissue, human thyroid cancer tissue, mouse ovary tissue, human breast cancer tissue, human cervical cancer tissue, human prostate cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SDF2L1 antibody was positively detected by IF in:
human breast cancer tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse
Immunofluorescence
5 μg/ml
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Dog
Predicted
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-SDF2L1 Antibody Picoband®
View all SDF2L1 Antibodies
View all SDF2L1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SDF2L1 recombinant protein (Position: A29-L221).
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
24 kDa
Calculated Molecular Weight
23.6 kDa
Background of SDF2L1
Enables misfolded protein binding activity. Involved in chaperone cofactor-dependent protein refolding. Located in endoplasmic reticulum. Part of chaperone complex.
Cite This Product
Anti-SDF2L1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13464)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Dog
98%
High Homology
Monkey
97%
High Homology
Rat
94%
High Homology
Mouse
92%
High Homology
Cow
92%
High Homology
Rabbit
91%
High Homology
Sheep
91%
High Homology
Zebrafish
70%
Moderate Homology
Drosophila
53%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SDF2L1 antibody
Immunohistochemistry (IHC) protocol for SDF2L1 antibody
Immunofluorescence (IF) protocol for SDF2L1 antibody
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Product Promise
We promise Anti-SDF2L1 Antibody Picoband® performs excellently in relevant applications for ELISA, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A13464
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026