Product Info Summary
SKU: | PA1839 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-SDHC Antibody
SKU/Catalog Number
PA1839
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SDHC Antibody catalog # PA1839. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SDHC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1839)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SDHC, different from the related mouse and rat sequences by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1839 is reactive to SDHC in Human, Mouse, Rat
Applications
PA1839 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
19 kDa
Calculated molecular weight
18.61kDa
Background of SDHC
SDHC (succinate dehydrogenase complex, subunit C, integral membrane protein, 15kDa) also known as SDH3, SUCCINATE DEHYDROGENASE CYTOCHROME b. The SDHC gene contains 6 exons. Oostveen et al. (1995) found that in fact it was protein from the bovine SDH3 gene (encoding 1 of the 2 integral membrane proteins) that complemented the hamster mutation. The authors localized the human SDH3 gene to the short arm of chromosome one, within 1 to 2 Mb from the centromere. Additionally, the authors stated that Southern analyses of human genomic DNA suggested that there are multiple SDH3 genes or pseudogenes. Elbehti-Green et al. (1998) confirmed the assignment of the SDHC gene to 1q21 by FISH. Baysal et al. (2004) described a family with PGL3 in which an 8,372-bp deletion in the SDHC gene was transmitted both maternally and paternally, without evidence of genomic imprinting.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Anti-SDHC antibody, PA1839, IHC(P)
IHC(P): Rat Gaster Tissue
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Anti-SDHC antibody, PA1839, IHC(P)
IHC(P): Human Gastric Cancer Tissue
Click image to see more details
Anti-SDHC antibody, PA1839, Western blotting
All lanes: Anti SDHC (PA1839) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 19KD
Observed bind size: 19KD
Protein Target Info & Infographic
Gene/Protein Information For SDHC (Source: Uniprot.org, NCBI)
Gene Name
SDHC
Full Name
Succinate dehydrogenase cytochrome b560 subunit, mitochondrial
Weight
18.61kDa
Superfamily
cytochrome b560 family
Alternative Names
Integral membrane protein CII-3; mitochondrial; PGL3; QPs1; QPs-1; SDH3; succinate dehydrogenase complex, subunit C, integral membrane protein, 15kD; succinate dehydrogenase complex, subunit C, integral membrane protein, 15kDa; succinate-ubiquinone oxidoreducatase cytochrome B large subunit SDHC CYB560, CYBL, PGL3, QPS1, SDH3 succinate dehydrogenase complex subunit C succinate dehydrogenase cytochrome b560 subunit, mitochondrial|cytochrome B large subunit of complex II|integral membrane protein CII-3b|large subunit of cytochrome b|succinate dehydrgenase cytochrome b|succinate dehydrogenase 3, integral membrane subunit|succinate dehydrogenase complex subunit C integral membrane protein 15kDa|succinate dehydrogenase complex, subunit C, integral membrane protein, 15kD|succinate-ubiquinone oxidoreductase cytochrome B large subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SDHC, check out the SDHC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SDHC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SDHC Antibody (PA1839)
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No publications found for PA1839
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-SDHC Antibody
Question
My question regards to test anti-SDHC antibody PA1839 on human right adrenal gland for research purposes, then I may be interested in using anti-SDHC antibody PA1839 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
The products we sell, including anti-SDHC antibody PA1839, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-05-08
Question
Does anti-SDHC antibody PA1839 work on horse WB with liver?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
Our lab technicians have not tested anti-SDHC antibody PA1839 on horse. You can run a BLAST between horse and the immunogen sequence of anti-SDHC antibody PA1839 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-23
Question
I have attached the WB image, lot number and protocol we used for right adrenal gland using anti-SDHC antibody PA1839. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-04
Question
Would PA1839 anti-SDHC antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-01
Answer
You can see on the product datasheet, PA1839 anti-SDHC antibody as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-01
Question
We are currently using anti-SDHC antibody PA1839 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-05
Answer
The anti-SDHC antibody (PA1839) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-05