Anti-SDHD Antibody

Product Name

Anti-SDHD Antibody

View all SDHD Antibodies

SKU/Catalog Number

A01261

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SDHD Antibody (Catalog # A01261). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SDHD antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-SDHD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01261)

Host

Rabbit

Contents

SDHD Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SDHD antibody was raised against a 15 amino acid synthetic peptide near the center of human SDHD. The immunogen is located within amino acids 30 - 80 of SDHD.

Cross-reactivity

At least four isoforms of SDHD are known to exist; this antibody will detect the two longest isoforms.

Reactive Species

A01261 is reactive to SDHD in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

17.0 kDa

Background of SDHD

The mitochondrial succinate dehydrogenase complex subunit D (SDHD) is one of four proteins that make up the tricarboxylic cycle enzyme succinate dehydrogenase (SCH). Studies have shown that mutations in SDHD often leads to hereditary paragangliomas, usually benign tumors of the autonomic nervous system, suggesting that SDHD also plays a role as a tumor-suppressor gene. In one family with a nonsense mutation (R22X) in the SDHD gene, a loss of heterozygosity was found in the paragangliomas, and within these tumors the enzymatic activity of Complex II in the mitochondrial respiratory chain was completely abolished. Furthermore, high levels of angiogenic factors EPAS1 and VEGF was observed, which may stimulate tumor growth.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01261 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SDHD antibody can be used for detection of SDHD by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information SDHD

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