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Product Info Summary
| SKU: | A07334-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-SEC61B Antibody Picoband®
SKU/Catalog Number
A07334-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SEC61B Antibody Picoband® catalog # A07334-2. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SEC61B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07334-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human SEC61B recombinant protein (Position: R16-S96). Human SEC61B shares 98.8% amino acid (aa) sequence identity with mouse SEC61B.
Reactive Species
A07334-2 is reactive to SEC61B in Human, Mouse, Rat
Observed Molecular Weight
15 kDa
Calculated molecular weight
10.0 kDa
Background of SEC61B
Protein transport protein Sec61 subunit beta is a protein that in humans is encoded by the SEC61B gene. The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07334-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg /1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human A431 whole cell, human PC-3 whole cell, human 293T whole cell, human Hela whole cell, rat C6 whole cell, mouse C2C12 whole cell, mouse RAW2647 whole cell, mouse 3T3-L1 whole cell
FCM: PC-3 cell
Validation Images & Assay Conditions
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Western blot analysis of SEC61B using anti-SEC61B antibody (A07334-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse C2C12 whole cell lysates,
Lane 7: mouse RAW264.7 whole cell lysates,
Lane 8: mouse 3T3-L1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC61B antigen affinity purified polyclonal antibody (Catalog # A07334-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC61B at approximately 15 kDa. The expected band size for SEC61B is at 10 kDa.
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Flow Cytometry analysis of PC-3 cells using anti-SEC61B antibody (A07334-2).
Overlay histogram showing PC-3 cells stained with A07334-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC61B Antibody (A07334-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-SEC61B Antibody Picoband® (A07334-2)
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