Anti-Secretagogin SCGN Antibody

Product Name

Anti-Secretagogin SCGN Antibody

View all Secretagogin Antibodies

SKU/Catalog Number

M10629-2

Size

100ul

Description

Boster Bio Anti-Secretagogin SCGN Antibody catalog # M10629-2. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Secretagogin SCGN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M10629-2)

Host

Chicken

Contents

Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN₃

Clonality

Polyclonal

Immunogen

Full-length recombinant human secretagogin protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M10629-2 is reactive to SCGN in Bovine, Human, Mouse, Rat

Applications

M10629-2 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

32.04kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1,000-5,000 IF/ICC or IHC: 1:1,000-1:2,000

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of tissue lysates using chicken pAb to secretagogin, M10629-2, dilution 1:1,000 in green: [1] protein standard, [2] mouse olfactory bulb [3] rat cerebellum, and [4] cow cerebellum. The band at ~27kDa corresponds to the secretagogin protein. Secretagogin protein is highly expressed in the mouse olfactory bulb, but significantly less in the cerebellum.

Click image to see more details

Immunofluorescent analysis of mouse brain olfactory bulb section stained with chicken pAb to secretagogin, M10629-2, dilution 1:1,000, in red, and costained with mouse mAb to calretinin, dilution 1:500 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45M, and free-floating sections were stained with the above antibodies. Secretagogin is highly expressed in the mitral and granular cell layers, while calretinin is in the glomerular layer. Cells that express secretagogin or calretinin only are red or green respectively. A number of cells containing both proteins appears orange-yellow. Mouse select image at left for larger view.

Gene/Protein Information For SCGN (Source: Uniprot.org, NCBI)

Alternative Names

CALBL; DJ501N12.8; SCGN; SECRET; Secretagogin; secretagogin, EF-hand calcium binding protein; SECRETsecretagogin; SEGN; Setagin SCGN CALBL, DJ501N12.8, SECRET, SEGN, setagin secretagogin, EF-hand calcium binding protein secretagogin

For more info on SCGN, check out the SCGN Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SCGN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the SCGN Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis