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Product Info Summary
| SKU: | M06559 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Semaphorin 3B/SEMA3B Antibody Picoband® (monoclonal, 9C4F7)
SKU/Catalog Number
M06559
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Semaphorin 3B/SEMA3B Antibody Picoband® (monoclonal, 9C4F7) catalog # M06559. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Semaphorin 3B/SEMA3B Antibody Picoband® (monoclonal, 9C4F7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06559)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
9C4F7
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human Semaphorin 3B/SEMA3B recombinant protein (Position: Y55-R624).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M06559 is reactive to SEMA3B in Human, Mouse, Rat
Observed Molecular Weight
83 kDa
Calculated molecular weight
83.1 kDa
Background of SEMA3B
Semaphorin-3Bis aproteinthat in humans is encoded by theSEMA3Bgene. It is mapped to 3p21.31. The semaphorin/collapsin family of molecules plays a critical role in the guidance of growth cones during neuronal development. The secreted protein encoded by this gene family member is important in axonal guidance and has been shown to act as a tumor suppressor by inducing apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06559 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human Hela whole cell, human CACO-2 whole cell, human U87 whole cell, human K562 whole cell, rat kidney tissue, rat NRK whole cell, mouse kidney tissue, mouse ANA-1 whole cell
ICC/IF: Hela cell
FCM: HepG2 cell
Validation Images & Assay Conditions
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Western blot analysis of Semaphorin 3B/SEMA3B using anti-Semaphorin 3B/SEMA3B antibody (M06559).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat NRK whole cell lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse ANA-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Semaphorin 3B/SEMA3B antigen affinity purified monoclonal antibody (Catalog # M06559) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Semaphorin 3B/SEMA3B at approximately 83 kDa. The expected band size for Semaphorin 3B/SEMA3B is at 83 kDa.
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IF analysis of Semaphorin 3B/SEMA3B using anti-Semaphorin 3B/SEMA3B antibody (M06559).
Semaphorin 3B/SEMA3B was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-Semaphorin 3B/SEMA3B Antibody (M06559) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Flow Cytometry analysis of HepG2 cells using anti-Semaphorin 3B/SEMA3B antibody (M06559).
Overlay histogram showing HepG2 cells stained with M06559 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Semaphorin 3B/SEMA3B Antibody (M06559, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-Semaphorin 3B/SEMA3B Antibody Picoband® (monoclonal, 9C4F7) (M06559)
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