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Serinc3 antibody (A05936-2) performs well in ELISA, Flow Cytometry, IF, ICC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of RAW264.7 cells using anti-SERINC3 antibody (A05936-2).
Overlay histogram showing RAW264.7 cells stained with A05936-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SERINC3 Antibody (A05936-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing RAW264.7 cells stained with A05936-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SERINC3 Antibody (A05936-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-SERINC3 Antibody Picoband®
Serinc3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, ICC, WB |
Tested Applications
Serinc3 antibody was positively detected by WB in:
rat liver tissue, rat kidney tissue, mouse liver tissue, human HEK293 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Serinc3 antibody was positively detected by ICC/IF in:
HEPA1-6 cell
Serinc3 antibody was positively detected by FC in:
RAW2647 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat
Immunocytochemistry/Immunofluorescence
5μg/ml
Mouse
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Mouse
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Rat
Tested
Recommend
Mouse
Tested
Recommend
Human
Tested
Product Information
Product Name
Anti-SERINC3 Antibody Picoband®
View all SERINC3 Antibodies
View all SERINC3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse SERINC3 recombinant protein (Position: E59-Y404).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
53 kDa
Calculated Molecular Weight
52.6 kDa
Background of Serinc3
Serine incorporator 3 is a protein that in humans is encoded by the SERINC3 gene. Also found on the membranes of the Golgi apparatus within cells, TDE1 is highly expressed in neuronal populations but is also found in thymus, kidney, liver and testis. Expression levels of TDE1 in tumors can be as much as tenfold the amount found in normal tissue of the same type. This increased expression implicates TDE1 as being involved in the cellular transformation from normal to malignant tissue. It is believed TDE1 contributes to oncogenesis by partially protecting cells from serum starvation and etoposide-induced apoptosis. The mechanism through which TDE1 protects cells is poorly understood, but may involve aberrant methylation of TDE1 complexes.
Cite This Product
Anti-SERINC3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05936-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
92%
High Homology
Rabbit
81%
Moderate Homology
Monkey
77%
Moderate Homology
Dog
77%
Moderate Homology
Human
76%
Moderate Homology
Cow
73%
Moderate Homology
Sheep
68%
Low homology
Zebrafish
58%
Low homology
Drosophila
42%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Serinc3 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for Serinc3 antibody
Flow Cytometry (FC) protocol for Serinc3 antibody
Review
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1 Review For Anti-SERINC3 Antibody Picoband®
This Antibody From Boster Bio Demonstrates Proficiency in Recognizing and Expressing the Protein Precisely
Source: Biocompare.com
| SKU | A05936-2 |
|---|---|
| Application | Western Blot |
| Sample | Ripa buffer lysis transfected HEK293 cell |
| Primary Incubation | +4°C overnight |
| Blocking Agent | 5% milk in 1X TBST |
| Secondary Incubation | 30 mins |
| Tertiary Incubation | 1:1000 |
| Detection | Clarity Western ECL Substrate |
| Results Summary | The antibody is excellent at recognizing and expressing the protein at the correct position. I have tried samples from different vendors, but only this vendor offers high-quality products. |
Zhannan Han, BME, Duke university, Lab Director / Man
Verified customer
Submitted 2025-02-08
Product Promise
We promise Anti-SERINC3 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A05936-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 31, 2026