Anti-Serine-protein kinase ATM ATM Antibody

Product Name

Anti-Serine-protein kinase ATM ATM Antibody

View all ATM Antibodies

SKU/Catalog Number

A00014

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Serine-protein kinase ATM ATM Antibody (Catalog # A00014). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

ATM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Serine-protein kinase ATM ATM Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00014)

Host

Rabbit

Contents

ATM Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ATM antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human ATM. Thae immunogen is located within amino acids 2970 - 3020 of ATM.

Reactive Species

A00014 is reactive to ATM in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

350.7 kDa

Background of ATM

DNA double strand breaks represent a major threat to an organism's genome. Eukaryotic cells have developed mechanisms that sense the presence this damage and initiate suitable responses that can include DNA repair, cell cycle delay, and programmed cell death. The ATM (mutated in Ataxia-Telangiectasia) protein kinase is activated following the formation of DNA double strand breaks, phosphorylating p53 and another kinase CHK2. This initiates a signaling cascade leading to the phosphorylation and inhibition of Cdc25, ultimately preventing cell cycle progression. In some cell types, such as the hemapoietic system, this leads to apoptosis instead of cell cycle arrest. Multiple isoforms of ATM are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00014 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ATM antibody can be used for detection of ATM by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ATM

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