Click image to see more details
Product Info Summary
| SKU: | A18879 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-SERPINE3 Antibody Picoband®
SKU/Catalog Number
A18879
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SERPINE3 Antibody Picoband® catalog # A18879. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SERPINE3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A18879)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SERPINE3 recombinant protein (Position: K90-F424).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A18879 is reactive to SERPINE3 in Human, Mouse, Rat
Observed Molecular Weight
52 kDa
Calculated molecular weight
47.0 kDa
Background of SERPINE3
Predicted to enable serine-type endopeptidase inhibitor activity. Predicted to be involved in negative regulation of endopeptidase activity. Predicted to be active in extracellular space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A18879 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of SERPINE3 using anti-SERPINE3 antibody (A18879).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINE3 antigen affinity purified polyclonal antibody (Catalog # A18879) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINE3 at approximately 52 kDa. The expected band size for SERPINE3 is at 47 kDa.
Click image to see more details
Temporal gene and protein expression changes after retinal detachment and sequencing validation. ( A ) Heatmap showing significantly upregulated (FC ≥ 1.5, p adj ≤0.05, red) and downregulated (FC ≤ 0.67, p adj ≤0.05, blue) DEGs 1 and 7 dprd. ( B-F ) Relative expression values of DEG grouped by temporal expression. ( B ) Log2FC of all DEGs significantly upregulated at both 1 and 7 dprd (Up/Up). ( C ) Log2FC of DEGs significantly upregulated only at 1 dprd (Up/NSC). Gray indicates DEG that were not significantly changed (NSC). ( D ) Log2FC of DEGs significantly upregulated only at 7 dprd (NSC/Up). ( E ) Log2FC of DEGs significantly downregulated only at 1 dprd (Down/NSC). ( F ) Log2FC of DEGs significantly downregulated at both 1 and 7 dprd (Down/Down). ( G-K ) QRT-PCR of temporal DEG expression changes after retinal detachment for RNA-Seq validation. Relative mRNA expression of ( G ) Grem1 , ( H ) S100a8 , ( I ) Serpine3 , ( J ) Rpe65 , and ( K ) Tsc22d3 , obtained from isolated naïve RPE ( n = 6), as well RD RPE at 1 dprd ( n = 6) and 7 dprd ( n = 6). Bar graphs represent mean ± SEM. Statistical analysis was performed using one-way ANOVA with repeated measures followed by Tukey’s post hoc test. * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001
Index in PubMed under a CC BY license. PMID: 38528525
Specific Publications For Anti-SERPINE3 Antibody Picoband® (A18879)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-SERPINE3 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-SERPINE3 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
