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Product Info Summary
| SKU: | M02211 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SET Monoclonal Antibody
SKU/Catalog Number
M02211
BM5570 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SET Monoclonal Antibody catalog # M02211. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SET Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02211)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEEO-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SET Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1.
Reactive Species
M02211 is reactive to SET in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
33.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02211 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:30
FC 1:20
Positive Control
WB: HepG2 cell
Validation Images & Assay Conditions
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Western blot analysis of SET expression in HepG2 cell lysate.
Specific Publications For Anti-SET Monoclonal Antibody (M02211)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-SET Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M02211?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of SET expression in HepG2 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
We are currently using anti-SET Monoclonal antibody M02211 for mouse tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
The anti-SET Monoclonal antibody (M02211) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-09
Question
Is a blocking peptide available for product anti-SET Monoclonal antibody (M02211)?
R. Miller
Verified customer
Asked: 2019-07-19
Answer
We do provide the blocking peptide for product anti-SET Monoclonal antibody (M02211). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-07-19
Question
Is there a BSA free version of anti-SET Monoclonal antibody M02211 available?
D. Huang
Verified customer
Asked: 2017-12-19
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-SET Monoclonal antibody M02211 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-19
Question
Will anti-SET Monoclonal antibody M02211 work for IP with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2017-06-12
Answer
According to the expression profile of cervix carcinoma erythroleukemia, SET is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-SET Monoclonal antibody M02211 will work for IP with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2017-06-12
Question
Would M02211 anti-SET Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Mitchell
Verified customer
Asked: 2015-05-14
Answer
You can see on the product datasheet, M02211 anti-SET Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-05-14
