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Product Info Summary
| SKU: | A00682 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Sex hormone-binding globulin SHBG Antibody
SKU/Catalog Number
A00682
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Sex hormone-binding globulin SHBG Antibody (Catalog # A00682). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Sex hormone-binding globulin SHBG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00682)
Host
Rabbit
Contents
SHBG antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SHBG antibody was raised against a 16 amino acid peptide near the center of human SHBG. The immunogen is located within amino acids 220 - 270 of SHBG.
Cross-reactivity
SHBG antibody is human, mouse, and rat reactive. At least three isoforms of SHBG are known to exist; this antibody will detect all three isoforms.
Reactive Species
A00682 is reactive to SHBG in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
43.8 kDa
Background of SHBG
SHBG is a steroid binding protein that was first described as a plasma protein secreted by the liver and is thought to participate in the regulation of steroid responses. SHBG transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers (1). Low plasma SHBG levels are associated with obesity, abdominal adiposity, metabolic syndrome, and predict the development of type 2 diabetes (2,3). Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00682 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SHBG antibody can be used for detection of SHBG by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SHBG in human liver tissue lysate with SHBG antibody at (A) 1 and (B) 2 μg/ml.
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Immunofluorescence of SHBG in mouse liver tissue with SHBG antibody at 20 μg/ml.
Specific Publications For Anti-Sex hormone-binding globulin SHBG Antibody (A00682)
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