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Product Info Summary
| SKU: | M02243 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SFPQ Monoclonal Antibody
SKU/Catalog Number
M02243
BM5546 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SFPQ Monoclonal Antibody catalog # M02243. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SFPQ Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02243)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AECI-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SFPQ DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts.
Reactive Species
M02243 is reactive to SFPQ in Human, Mouse, Rat
Observed Molecular Weight
100 kDa
Calculated molecular weight
76.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02243 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: A431 cell
Validation Images & Assay Conditions
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Western blot analysis of SFPQ expression in A431 cell lysate.
Specific Publications For Anti-SFPQ Monoclonal Antibody (M02243)
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4 Customer Q&As for Anti-SFPQ Monoclonal Antibody
Question
We are currently using anti-SFPQ Monoclonal antibody M02243 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
The anti-SFPQ Monoclonal antibody (M02243) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-05
Question
I was wanting to use your anti-SFPQ Monoclonal antibody for IF for mouse leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
It shows on the product datasheet, M02243 anti-SFPQ Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-15
Question
What sample was tested in WB and IHC applications with M02243?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of SFPQ expression in A431 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
Will anti-SFPQ Monoclonal antibody M02243 work for IF with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
According to the expression profile of leukemic t-cell, SFPQ is highly expressed in leukemic t-cell. So, it is likely that anti-SFPQ Monoclonal antibody M02243 will work for IF with leukemic t-cell.
Boster Scientific Support
Answered: 2019-06-03
