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Product Info Summary
| SKU: | A07683-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-SFR1 Antibody Picoband®
SKU/Catalog Number
A07683-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SFR1 Antibody Picoband® catalog # A07683-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SFR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07683-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SFR1.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07683-1 is reactive to SFR1 in Human, Mouse, Rat
Observed Molecular Weight
22 kDa
Calculated molecular weight
28.3 kDa
Background of SFR1
SFR1 is a component of the SWI5-SFR1 complex, a complex required for double-strand break repair via homologous recombination.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07683-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Positive Control
WB: human A431 whole cell, human U251 whole cell, human Hacat whole cell, human Jurkat whole cell, rat liver tissue, mouse brain tissue, mouse liver tissue
FCM: K562 cell, U251 cell
Validation Images & Assay Conditions
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Western blot analysis of SFR1 using anti-SFR1 antibody (A07683-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFR1 antigen affinity purified polyclonal antibody (Catalog # A07683-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFR1 at approximately 22 kDa. The expected band size for SFR1 is at 28 kDa.
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Flow Cytometry analysis of U251 cells using anti-SFR1 antibody (A07683-1).
Overlay histogram showing U251 cells stained with A07683-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFR1 Antibody (A07683-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-SFR1 Antibody Picoband® (A07683-1)
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