Product Info Summary
SKU: | A10224-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-SFRS15 (V641) SCAF4 Antibody
SKU/Catalog Number
A10224-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SFRS15 (V641) SCAF4 Antibody catalog # A10224-2. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SFRS15 (V641) SCAF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10224-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human SFRS15.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10224-2 is reactive to SCAF4 in Human, Mouse, Rat
Applications
A10224-2 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
125.869kDa
Background of SFRS15
May act to physically and functionally link transcription and pre-mRNA processing By similarity.
Tassone F., Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases.
Hattori M., Nature 405:311-319(2000).
Hirosawa M., DNA Res. 6:329-336(1999).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of SCAF4 using Anti-SFRS15 (V641) SCAF4 Antibody (A10224-2).
Immunohistochemistry (IHC) analyzes of SFRS15 (V641) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-SFRS15 (V641) SCAF4 Antibody A10224-2
Western blot (WB) analysis of SFRS15 (V641) pAb at 1:1000 dilutionLane1:C6 whole cell lysate(40ug)
Lane2:CT26 whole cell lysate(40ug)
Lane3:HCT116 whole cell lysate(40ug)
Lane4:SGC7901 whole cell lysate(40ug)
Lane5:HEK293T whole cell lysate(20ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For SCAF4 (Source: Uniprot.org, NCBI)
Gene Name
SCAF4
Full Name
SR-related and CTD-associated factor 4
Weight
125.869kDa
Alternative Names
CTD-binding SR-like protein RA4; FLJ23364; KIAA1172arginine/serine-rich 15; SFRS15; SR-like CTD-associated factor 4; SR-related and CTD-associated factor 4; SR-related CTD-associated factor 4 SCAF4 SFRS15, SRA4 SR-related CTD associated factor 4 SR-related and CTD-associated factor 4|CTD-binding SR-like protein rA4|SR-like CTD-associated factor 4|pre-mRNA splicing SR protein rA4|splicing factor serine alanine 15|splicing factor, arginine/serine-rich 15
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SCAF4, check out the SCAF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SCAF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SFRS15 (V641) SCAF4 Antibody (A10224-2)
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No publications found for A10224-2
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