Anti-Prosurfactant Protein B/SFTPB Antibody Picoband™

Product Name

Anti-Prosurfactant Protein B/SFTPB Antibody Picoband™

View all SP-B/Surfactant Protein B Antibodies

SKU/Catalog Number

A03441-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Prosurfactant Protein B/SFTPB Antibody Picoband™ catalog # A03441-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Prosurfactant Protein B/SFTPB Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03441-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SFTPB, which shares 80% amino acid (aa) sequence identity with both mouse and rat SFTPB.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03441-1 is reactive to SFTPB in Human, Mouse, Rat

Applications

A03441-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

46 kDa

Calculated molecular weight

42.117kDa

Background of SP-B/Surfactant Protein B

Pulmonary surfactant-associated protein B is a protein that in humans is encoded by the SFTPB gene. This gene encodes the pulmonary-associated surfactant protein B (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period. Alternatively spliced transcript variants encoding the same protein have been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of SFTPB using anti-SFTPB antibody (A03441-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human COLO-320 whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPB antigen affinity purified polyclonal antibody (Catalog # A03441-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFTPB at approximately 46KD. The expected band size for SFTPB is at 42KD.

Gene/Protein Information For SFTPB (Source: Uniprot.org, NCBI)

Alternative Names

18 kDa pulmonary-surfactant protein; 6 kDa protein; PSP-B; pulmonary surfactant-associated protein B; Pulmonary surfactant-associated proteolipid SPL(Phe); SFTB3; SFTP3; SFTPB; SMDP1; SP-B surfactant, pulmonary-associated protein B; SP-B; surfactant protein B SFTPB PSP-B, SFTB3, SFTP3, SMDP1, SP-B surfactant protein B pulmonary surfactant-associated protein B|18 kDa pulmonary-surfactant protein|6 kDa protein|pulmonary surfactant-associated proteolipid SPL(Phe)

For more info on SFTPB, check out the SFTPB Infographic

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In this infographic, you will see the following information for SFTPB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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