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Product Info Summary
| SKU: | PB9434 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SGK1 Antibody Picoband®
SKU/Catalog Number
PB9434
PB0434 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SGK1 Antibody Picoband® catalog # PB9434. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SGK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9434)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SGK1, different from the related mouse sequence by three amino acids, and from the related rat sequence by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9434 is reactive to SGK1 in Human
Observed Molecular Weight
49 kDa
Calculated molecular weight
48.9 kDa
Background of SGK1
Serine/threonine-protein kinase Sgk1, also called SGK1, encodes a serine/threonine protein kinase that plays an important role in cellular stress response. By fluorescence in situ hybridization, this gene is mapped to 6q23.2. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9434 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HEPG2 Whole Cell
Validation Images & Assay Conditions
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Western blot analysis of SGK1 using anti-SGK1 antibody (PB9434).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: HEPG2 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGK1 antigen affinity purified polyclonal antibody (Catalog # PB9434) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGK1 at approximately 49 kDa. The expected band size for SGK1 is at 49 kDa.
Specific Publications For Anti-SGK1 Antibody Picoband® (PB9434)
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4 Customer Q&As for Anti-SGK1 Antibody Picoband®
Question
you antibody using your anti-SGK1 antibody for positive regulation of transporter activity studies. Has this antibody been tested with western blotting on hepg2 whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
Thanks for your inquiry. This PB9434 anti-SGK1 antibody is validated on hepg2 whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-12
Question
We are currently using anti-SGK1 antibody PB9434 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-29
Answer
The anti-SGK1 antibody (PB9434) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-29
Question
We have observed staining in human female gonad. What should we do? Is anti-SGK1 antibody supposed to stain female gonad positively?
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
From what I have seen in literature female gonad does express SGK1. From what I have seen in Uniprot.org, SGK1 is expressed in female gonad, brain, glioblastoma, hippocampus skin, hair follicle dermal papilla, cervix, cervix carcinoma, among other tissues. Regarding which tissues have SGK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10191262, 14702039, 18753299
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976, 20068231
Boster Scientific Support
Answered: 2018-11-23
Question
Our lab were well pleased with the WB result of your anti-SGK1 antibody. However we have seen positive staining in hair follicle dermal papilla cytoplasm. nucleus. endoplasmic reticulum using this antibody. Is that expected? Could you tell me where is SGK1 supposed to be expressed?
G. Krishna
Verified customer
Asked: 2018-08-03
Answer
According to literature, hair follicle dermal papilla does express SGK1. Generally SGK1 expresses in cytoplasm. nucleus. endoplasmic reticulum, isoform 2: cell membrane. Regarding which tissues have SGK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10191262, 14702039, 18753299
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976, 20068231
Boster Scientific Support
Answered: 2018-08-03
