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Product Info Summary
| SKU: | M00673 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-SGK1/Sgk Rabbit Monoclonal Antibody
SKU/Catalog Number
M00673
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SGK1/Sgk Rabbit Monoclonal Antibody catalog # M00673. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SGK1/Sgk Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00673)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EHF-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SGK1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00673 is reactive to SGK1 in Human, Mouse, Rat
Applications
M00673 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Observed Molecular Weight
30 kDa
Calculated molecular weight
48.942kDa
Background of SGK1
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
IP 1:50
FC 1:30
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human lung cancer, using SGK1 Antibody.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of SGK1 expression in A431 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For SGK1 (Source: Uniprot.org, NCBI)
Gene Name
SGK1
Full Name
Serine/threonine-protein kinase Sgk1
Weight
48.942kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.1; serine/threonine protein kinase SGK; serine/threonine-protein kinase Sgk1; serum/glucocorticoid regulated kinase 1; serum/glucocorticoid regulated kinase; Serum/glucocorticoid-regulated kinase 1; SGK; SGK1 SGK1 SGK serum/glucocorticoid regulated kinase 1 serine/threonine-protein kinase Sgk1|Sgk1 variant i3|serine/threonine protein kinase SGK
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SGK1, check out the SGK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SGK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SGK1/Sgk Rabbit Monoclonal Antibody (M00673)
Hello CJ!
M00673 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Scortegagna M1, Berthon A2, Settas N2, Giannakou A2, Garcia G1, Li JL1, James B1, Liddington RC1, Vilches-Moure JG3, Stratakis CA2, Ronai ZA1,4. JCI Insight. 2017 Dec 7;2(23). pii: 97128. doi: 10.1172/jci.insight.97128. [Epub ahead of print] The E...
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-SGK1/Sgk Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-SGK1/Sgk Rabbit Monoclonal antibody (M00673)?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
We do provide the blocking peptide for product anti-SGK1/Sgk Rabbit Monoclonal antibody (M00673). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-21
Question
Does anti-SGK1/Sgk Rabbit Monoclonal antibody M00673 work for IHC with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
According to the expression profile of cervix carcinoma, SGK1 is highly expressed in cervix carcinoma. So, it is likely that anti-SGK1/Sgk Rabbit Monoclonal antibody M00673 will work for IHC with cervix carcinoma.
Boster Scientific Support
Answered: 2020-03-04
Question
I see that the anti-SGK1/Sgk Rabbit Monoclonal antibody M00673 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-30
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma using anti-SGK1/Sgk Rabbit Monoclonal antibody M00673. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-10
Question
Do you have a BSA free version of anti-SGK1/Sgk Rabbit Monoclonal antibody M00673 available?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SGK1/Sgk Rabbit Monoclonal antibody M00673 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-09
Question
I was wanting to use your anti-SGK1/Sgk Rabbit Monoclonal antibody for IHC for rat cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
As indicated on the product datasheet, M00673 anti-SGK1/Sgk Rabbit Monoclonal antibody has been tested for Flow Cytometry, IP, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-SGK1/Sgk Rabbit Monoclonal antibody M00673. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-18
Question
We have been able to see staining in mouse female gonad. What should we do? Is anti-SGK1/Sgk Rabbit Monoclonal antibody supposed to stain female gonad positively?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
According to literature female gonad does express SGK1. According to Uniprot.org, SGK1 is expressed in female gonad, brain, glioblastoma, hippocampus skin, hair follicle dermal papilla, cervix, cervix carcinoma, among other tissues. Regarding which tissues have SGK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10191262, 14702039, 18753299
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976, 20068231
Boster Scientific Support
Answered: 2019-08-27
Question
Will M00673 anti-SGK1/Sgk Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
As indicated on the product datasheet, M00673 anti-SGK1/Sgk Rabbit Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-26
Question
Would anti-SGK1/Sgk Rabbit Monoclonal antibody M00673 work on primate WB with hair follicle dermal papilla?
A. Edwards
Verified customer
Asked: 2019-02-15
Answer
Our lab technicians have not tested anti-SGK1/Sgk Rabbit Monoclonal antibody M00673 on primate. You can run a BLAST between primate and the immunogen sequence of anti-SGK1/Sgk Rabbit Monoclonal antibody M00673 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate hair follicle dermal papilla in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-15
Question
We are interested in using your anti-SGK1/Sgk Rabbit Monoclonal antibody for regulation of tp53 degradation studies. Has this antibody been tested with western blotting on a431 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-10-01
Answer
We appreciate your inquiry. This M00673 anti-SGK1/Sgk Rabbit Monoclonal antibody is tested on a431 cell lysate. It is guaranteed to work for Flow Cytometry, IP, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-10-01
Question
My boss were content with the WB result of your anti-SGK1/Sgk Rabbit Monoclonal antibody. However we have been able to see positive staining in glioblastoma isoform 2: cell membrane. using this antibody. Is that expected? Could you tell me where is SGK1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-12-29
Answer
From what I have seen in literature, glioblastoma does express SGK1. Generally SGK1 expresses in cytoplasm. nucleus. endoplasmic reticulum, isoform 2: cell membrane. Regarding which tissues have SGK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10191262, 14702039, 18753299
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976, 20068231
Boster Scientific Support
Answered: 2017-12-29
Question
Is this M00673 anti-SGK1/Sgk Rabbit Monoclonal antibody reactive to the isotypes of SGK1?
Verified Customer
Verified customer
Asked: 2017-12-01
Answer
The immunogen of M00673 anti-SGK1/Sgk Rabbit Monoclonal antibody is A synthesized peptide derived from human SGK1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-01
Question
We ordered your anti-SGK1/Sgk Rabbit Monoclonal antibody for IHC on female gonad a few months ago. I am using human, and I plan to use the antibody for WB next. My question regards examining female gonad as well as hair follicle dermal papilla in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-09-05
Answer
I took a look at the website and datasheets of our anti-SGK1/Sgk Rabbit Monoclonal antibody and it seems that M00673 has been validated on human in both IHC and WB. Thus M00673 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-09-05
Question
I am interested in to test anti-SGK1/Sgk Rabbit Monoclonal antibody M00673 on rat cervix carcinoma for research purposes, then I may be interested in using anti-SGK1/Sgk Rabbit Monoclonal antibody M00673 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Jones
Verified customer
Asked: 2017-07-06
Answer
The products we sell, including anti-SGK1/Sgk Rabbit Monoclonal antibody M00673, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-06
Question
We are currently using anti-SGK1/Sgk Rabbit Monoclonal antibody M00673 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
J. Yang
Verified customer
Asked: 2016-12-13
Answer
The anti-SGK1/Sgk Rabbit Monoclonal antibody (M00673) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-12-13
Question
My question regarding product M00673, anti-SGK1/Sgk Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
F. Parker
Verified customer
Asked: 2014-09-17
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00673 anti-SGK1/Sgk Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-09-17
