Anti-SGLT1/SLC5A1 Antibody

Product Name

Anti-SGLT1/SLC5A1 Antibody

View all SGLT1/SLC5A1 Antibodies

SKU/Catalog Number

PA2244

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SGLT1/SLC5A1 Antibody catalog # PA2244. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SGLT1/SLC5A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2244)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse SGLT1, different from the related rat sequence by one amino acid, and from the related human sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2244 is reactive to SLC5A1 in Human, Mouse, Rat

Applications

PA2244 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

73 kDa

Calculated molecular weight

73.498kDa

Background of SGLT1/SLC5A1

Sodium/glucose cotransporter 1 (SLC5A1), also known as NAGT or SGLT1 is a protein that in humans is encoded by the SLC5A1 gene. This gene is mapped to 22q12.3. This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SLC5A1 using anti-SLC5A1 antibody (PA2244).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat NRK whole cell lysates,
Lane 5: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A1 antigen affinity purified polyclonal antibody (Catalog # PA2244) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC5A1 at approximately 73 kDa. The expected band size for SLC5A1 is at 73 kDa.

Gene/Protein Information For SLC5A1 (Source: Uniprot.org, NCBI)

Alternative Names

D22S675; High affinity sodium-glucose cotransporter; Na+/glucose cotransporter 1; NAGT; NAGTsodium/glucose cotransporter 1; SGLT1; SGLT1Na(+)/glucose cotransporter 1; SLC5A1; solute carrier family 5 (sodium/glucose cotransporter), member 1; Solute carrier family 5 member 1 SLC5A1 D22S675, NAGT, SGLT1 solute carrier family 5 member 1 sodium/glucose cotransporter 1|Na+/glucose cotransporter 1|high affinity sodium-glucose cotransporter|solute carrier family 5 (sodium/glucose cotransporter), member 1

For more info on SLC5A1, check out the SLC5A1 Infographic

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In this infographic, you will see the following information for SLC5A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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