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Product Info Summary
| SKU: | A12048 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-SGSM1 Antibody
SKU/Catalog Number
A12048
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SGSM1 Antibody (Catalog # A12048). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SGSM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SGSM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12048)
Host
Rabbit
Contents
SGSM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SGSM1 antibody was raised against a 17 amino acid synthetic peptide near the center of human SGSM1. The immunogen is located within amino acids 480 - 530 of SGSM1.
Cross-reactivity
At least four isoforms of SGSM1 are known to exist; SGSM1 antibody will detect all four isoforms.
Reactive Species
A12048 is reactive to SGSM1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
129.7 kDa
Background of SGSM1
Small G proteins such as RAP and RAB proteins are the key molecules in intracellular signal transduction and vesicle transportation. A novel protein family small G protein signaling modulator (SGSM) consisting of three members SGSM1-3 bind to RAP and RAB family proteins. All three SGSM proteins possess both a RUN domain and a TBC domain. SGSM1 (RUTBC2) is a 1,148 amino acid protein that localizes to the Golgi apparatus and is mainly expressed in the CNS. SGSM1 interacts with RAP and RAB subfamily members of the small G proteins, and function as modulators of RAP and RAB-mediated neuronal signal transduction and vesicular transportation pathways.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12048 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SGSM1 antibody can be used for detection of SGSM1 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SGSM1 in human cerebellum tissue lysate with SGSM1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunofluorescence of SGSM1 in human brain tissue with SGSM1 antibody at 20 μg/mL.
Specific Publications For Anti-SGSM1 Antibody (A12048)
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