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Product Info Summary
| SKU: | A13056-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SGT2/SGTB Antibody Picoband®
SKU/Catalog Number
A13056-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SGT2/SGTB Antibody Picoband® catalog # A13056-1. Tested in ELISA, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SGT2/SGTB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13056-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SGT2/SGTB recombinant protein (Position: Q20-A268).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A13056-1 is reactive to SGTB in Human, Rat
Observed Molecular Weight
36 kDa
Calculated molecular weight
33.4 kDa
Background of SGTB
Predicted to enable molecular adaptor activity. Predicted to be involved in positive regulation of chaperone-mediated protein folding; posttranslational protein targeting to endoplasmic reticulum membrane; and ubiquitin-dependent ERAD pathway. Predicted to be part of TRC complex. Predicted to be active in membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13056-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HepG2 whole cell, rat brain tissue
Validation Images & Assay Conditions
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Western blot analysis of SGT2/SGTB using anti-SGT2/SGTB antibody (A13056-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde
r reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGT2/SGTB antigen affinity purified polyclonal antibody (Catalog # A13056-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGT2/SGTB at approximately 36 kDa. The expected band size for SGT2/SGTB is at 34 kDa.
Specific Publications For Anti-SGT2/SGTB Antibody Picoband® (A13056-1)
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