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Product Info Summary
| SKU: | A09036 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-SH3 domain-binding protein 4 SH3BP4 Antibody
SKU/Catalog Number
A09036
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SH3 domain-binding protein 4 SH3BP4 Antibody (Catalog # A09036). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SH3BP4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SH3 domain-binding protein 4 SH3BP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09036)
Host
Rabbit
Contents
SH3BP4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SH3BP4 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human SH3BP4. The immunogen is located within amino acids 860 - 910 of SH3BP4.
Reactive Species
A09036 is reactive to SH3BP4 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
107.5 kDa
Background of SH3BP4
SH3BP4 was initially isolated as a novel cDNA using differential display analysis using cultured human corneal fibroblasts. The protein contains three Asn-Pro-Phe (NPF) motifs, an SH3 domain, a PXXP motif, a bipartite nuclear targeting signal, and a tyrosine phosphorylation site. SH3BP4 has been shown to interact with specific endocytic proteins such as clathrin, dynamin, and the transferrin receptor (TfR) and localizes to TfR-containing coated pits and vesicles. It is thought to be involved in cargo-specific control of clathrin-mediated endocytosis, specifically controlling the internalization of TfR. At least two isoforms of SH3BP4 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09036 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Recommend Dilution
SH3BP4 antibody can be used for detection of SH3BP4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in rat samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SH3BP4 in rat lung tissue lysate with SH3BP4 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of SH3BP4 in human lung tissue with SH3BP4 antibody at 5 μg/mL.
Specific Publications For Anti-SH3 domain-binding protein 4 SH3BP4 Antibody (A09036)
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