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Product Info Summary
| SKU: | A14323 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-SIGLEC15 Antibody
SKU/Catalog Number
A14323
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SIGLEC15 Antibody (Catalog # A14323). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SIGLEC15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SIGLEC15 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14323)
Host
Rabbit
Contents
SIGLEC15 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SIGLEC15 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human SIGLEC15. The immunogen is located within amino acids 50 - 100 of SIGLEC15.
Cross-reactivity
SIGLEC15 antibody is predicted to not cross-react with other SIGLEC family members.
Reactive Species
A14323 is reactive to SIGLEC15 in Human, Mouse, Rat
Observed Molecular Weight
45 kDa
Calculated molecular weight
35.7 kDa
Background of SIGLEC15
Siglecs are vertebrate cell-surface lectins that recognize sialylated glycans and are involved in many physiological processes, such as glycoprotein turnover, cellular trafficking, and pathogen recognition. Most Siglecs are expressed on cells of the immune system. SIGLEC15 is a type-I transmembrane protein consisting of two immunoglobulin (Ig)-like domains, a transmembrane domain containing a lysine residue, and a short cytoplasmic tail. SIGLEC15 can interact with the activating adaptor molecules DAP12/10. Its activating signaling potential and unique preference for glycan recognition implies that SIGLEC15 may be involved in the immune surveillance of tumors and probably plays a conserved, regulatory role in the immune system of vertebrates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14323 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-4 μg/mL; IHC-P/IF: 5-20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in the human, mouse and rat samples. All other applications and species not yet tested.
Validation Images & Assay Conditions
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Western blot analysis of SIGLEC15 in human kidney tissue lysate with SIGLEC15 antibody at (A) 1 and (B) 2 μg/mL.
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WB Validation in Human Tissues Loading: 15 μg of lysate Antibodies: SIGLEC15, A14323, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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WB Validation in Mouse Kidney Loading: 15 μg of lysate Antibodies: SIGLEC15, A14323, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000
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WB Validation in Rat Testis Loading: 15 μg of lysate Antibodies: SIGLEC15, A14323, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunofluorescence Validation of SIGLEC15 in Human Spleen Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling SIGLEC15 with A14323 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
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Immunofluorescence Validation of SIGLEC15 in Mouse Spleen Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse spleen tissue labeling SIGLEC15 with A14323 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
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Immunofluorescence Validation of SIGLEC15 in Rat Small Intestine Immunofluorescent analysis of 4% paraformaldehyde-fixed rat small intestine tissue labeling SIGLEC15 with A14323 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Specific Publications For Anti-SIGLEC15 Antibody (A14323)
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