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Product Info Summary
| SKU: | A04399 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-SIK1 Antibody
SKU/Catalog Number
A04399
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SIK1 Antibody (Catalog # A04399). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human.
Storage & Handling
SIK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SIK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04399)
Host
Rabbit
Contents
SIK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SIK1 antibody was raised against an 18 amino acid synthetic peptide near the center of human SIK1. The immunogen is located within amino acids 340 - 390 of SIK1.
Cross-reactivity
Two isoforms of SIK1 are known to exist; this antibody will detect both isoforms. SIK1 antibody is predicted to not cross-react with other SIK protein family members.
Reactive Species
A04399 is reactive to SIK1 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
84.9 kDa
Background of SIK1
Salt-inducible kinase 1 (SIK1), also known as SNF1LK or MSK, plays a role in histone modification and G2/M cell cycle regulation. It is a 783 amino acid protein that contains one UBA domain and belongs to the Ser/Thr protein kinase family (AMPK subfamily). Localized to both the nucleus and the cytoplasm, SIK1 is a class II HDAC kinase that uses magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins and is thought to be important for the early stages of skeletal muscle growth and myocardial cell differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04399 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SIK1 antibody can be used for detection of SIK1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SIK1 in human small intestine tissue lysate with SIK1 antibody at 1 μg/mL.
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Immunohistochemistry of SIK1 in human small intestine tissue with SIK1 antibody at 5 μg/mL.
Specific Publications For Anti-SIK1 Antibody (A04399)
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