Anti-SIRP alpha Antibody

SIRP alpha/CD172a antibody

Boster Bio Anti-SIRP alpha Antibody (Catalog # A05151). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A05151
Size: 0.1 mg
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, ICC, WB

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Product Name

Anti-SIRP alpha Antibody

View all SIRP alpha/CD172a Antibodies

SKU/Catalog Number

A05151

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SIRP alpha Antibody (Catalog # A05151). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SIRP alpha antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-SIRP alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05151)

Host

Rabbit

Contents

SIRP alpha Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

SK7

Isotype

IgG

Immunogen

SIRP alpha antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human SIRP alpha 1. The immunogen is located within the last 50 amino acids of SIRP alpha.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Recognizes SIRP alpha 1, 2 and 3.

Reactive Species

A05151 is reactive to Sirpa in Human, Mouse, Rat

Applications

A05151 is guaranteed for ELISA, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

56.414kDa

Background of SIRP alpha/CD172a

Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPalpha, SHPS-1, MyD-1, BIT and p84. SIRPalpha is a new gene family containing at least fifteen members. SIRPalpha is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2. SIRPalpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SIRP alpha antibody can be used for Western blot at 0.5 - 1 μg/mL. 75 - 110 kDa bands should be detected. Antibody can also be used for immunocytochemistry starting at 1 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For Sirpa (Source: Uniprot.org, NCBI)

Gene Name

Sirpa

Full Name

Tyrosine-protein phosphatase non-receptor type substrate 1

Weight

56.414kDa

Alternative Names

BIT; BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs; Brain Ig-like molecule with tyrosine-based activation motifs; CD172 antigen-like family member A; CD172a antigen; CD172a; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MFR; MFRtyrosine phosphatase SHP substrate 1; MyD-1 antigen; MYD1; MYD-1; P84; protein tyrosine phosphatase, non-receptor type substrate 1; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; signal-regulatory protein alpha; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; SIRP alph Sirpa|AI835480, Bi, Bit, CD172, CD172a, P8, P84, Pt, Ptpns1, SHP, SHP-1, SHPS-1, SIRP|signal-regulatory protein alpha|tyrosine-protein phosphatase non-receptor type substrate 1|CD172 antigen-like family member A|SHP substrate 1|brain Ig-like molecule with tyrosine-based activation motifs|brain immunological-like with tyrosine-based motifs|inhibitory receptor SHPS-1|mSIRP-alpha1|myD-1 antigen|protein tyrosine phosphatase, non-receptor type substrate 1|signal-regulatory protein alpha-1|sirp-alpha-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Sirpa, check out the Sirpa Infographic

Sirpa infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Sirpa: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A05151-0.1 mg

0.1 mg

$449

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