Anti-SIVA/SIVA1 Antibody Picoband™

Product Name

Anti-SIVA/SIVA1 Antibody Picoband™

View all SIVA Antibodies

SKU/Catalog Number

A05419-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SIVA/SIVA1 Antibody Picoband™ catalog # A05419-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SIVA/SIVA1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05419-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SIVA/SIVA1 recombinant protein (Position: M1-T141).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05419-1 is reactive to SIVA1 in Human

Applications

A05419-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

19 kDa

Calculated molecular weight

18.695kDa

Background of SIVA

Apoptosis regulatory protein Siva is a protein that in humans is encoded by the SIVA1 gene. This gene encodes an E3 ubiquitin ligase that regulates cell cycle progression, cell proliferation and apoptosis. The N-terminus of this protein binds to the cytoplasmic tail of the CD27 antigen, a member of the tumor necrosis factor receptor (TNFR) superfamily. In response to UV radiation-induced DNA damage, this protein has been shown to mediate the ubiquitination of proliferating cell nuclear antigen (PCNA), an important step in translesion DNA synthesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SIVA/SIVA1 using anti-SIVA/SIVA1 antibody (A05419-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde
r reducing conditions.
Lane 1: human MOLT-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIVA/SIVA1 antigen affinity purified polyclonal antibody (Catalog # A05419-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIVA/SIVA1 at approximately 19 kDa. The expected band size for SIVA/SIVA1 is at 19 kDa.

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Figure 2. Flow Cytometry analysis of U937 cells using anti-SIVA/SIVA1 antibody (A05419-1).
Overlay histogram showing U937 cells stained with A05419-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIVA/SIVA1 Antibody (A05419-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Figure 3. Flow Cytometry analysis of PC-3 cells using anti-SIVA/SIVA1 antibody (A05419-1).
Overlay histogram showing PC-3 cells stained with A05419-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIVA/SIVA1 Antibody (A05419-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SIVA1 (Source: Uniprot.org, NCBI)

Alternative Names

CD27-binding (Siva) protein; CD27BPCD27-binding protein; Siva-1; SIVA1, apoptosis-inducing factor; Siva-2; SIVAapoptosis regulatory protein Siva SIVA1 CD27BP, SIVA, Siva-1, Siva-2 SIVA1 apoptosis inducing factor apoptosis regulatory protein Siva|CD27-binding (Siva) protein

For more info on SIVA1, check out the SIVA1 Infographic

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In this infographic, you will see the following information for SIVA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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