Product Info Summary
SKU: | A00476 |
---|---|
Size: | 100uL |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IP |
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Product info
Product Name
Anti-SKP1 Antibody
SKU/Catalog Number
A00476
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-SKP1 Antibody (Catalog # A00476). Tested in IP applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-SKP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00476)
Host
Rabbit
Contents
0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Clone Number
8F6A9; 8H5C5
Isotype
Antiserum
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 152-163 of Human SKP1 (C-terminus) coupled to KLH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00476 is reactive to SKP1 in Human
Applications
A00476 is guaranteed for IP Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
18.658kDa
Background of SKP1
SKP1 is also known as S-phase kinase-associated protein 1A, Cyclin A/CDK2-associated protein p19, p19A, p19skp1, RNA polymerase II elongation factor-like protein, Organ of Corti protein 2, OCP-II protein, OCP-2, Transcription elongation factor B, and SIII. SKP1 is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, SKP1 serves as an adapter that links the F-box protein to CUL1. SKP1 interacts directly with CUL1 and F-box proteins, such as BTRC and SKP2, in the SCF complex. SKP1 also interacts with FBX29 and the cyclin A/CDK2 complex. SKP1 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1 and FBXW8 and is also a component of a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1A, APC and TBL1X.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:2,000 - 1:10,000
IHC: User optimized
WB: 1:500 - 1:1,000
Validation Images & Assay Conditions
Click image to see more details
Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.Data contributed by S.Jentsch.
Protein Target Info & Infographic
Gene/Protein Information For SKP1 (Source: Uniprot.org, NCBI)
Gene Name
SKP1
Full Name
S-phase kinase-associated protein 1
Weight
18.658kDa
Superfamily
SKP1 family
Alternative Names
Cyclin-A/CDK2-associated protein p19; EMC19p19skp1; MGC34403; OCP2; OCP2OCP-2; OCP-IIS-phase kinase-associated protein 1A (p19A); Organ of Corti protein 2; Organ of Corti protein II; p19a; p19Acyclin A/CDK2-associated p19; p19Skp1; RNA polymerase II elongation factor-like protein OCP2; RNA polymerase II elongation factor-like protein; SKP1; SKP1A; SKP1Acyclin A/CDK2-associated protein p19; S-phase kinase-associated protein 1; TCEB1LSIII; Transcription elongation factor B SKP1 EMC19, OCP-II, OCP2A, TCEB1L, p19A, SKP1 S-phase kinase associated protein 1 S-phase kinase-associated protein 1|OCP-2|RNA polymerase II elongation factor-like protein OCP2|SIII|cyclin A/CDK2-associated p19|cyclin-A/CDK2-associated protein p19|organ of Corti protein 2|organ of Corti protein II|p19skp1|transcription elongation factor B polypeptide 1-like
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SKP1, check out the SKP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SKP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SKP1 Antibody (A00476)
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No publications found for A00476
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