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Product Info Summary
| SKU: | A03988-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC |
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Product info
Product Name
Anti-SLAM/CD150/SLAMF1 Antibody
SKU/Catalog Number
A03988-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SLAM/CD150/SLAMF1 Antibody catalog # A03988-2. Tested in ELISA, Flow Cytometry, IHC applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SLAM/CD150/SLAMF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03988-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SLAM/CD150/SLAMF1 recombinant protein (Position: K34-N213).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03988-2 is reactive to SLAMF1 in Human
Calculated molecular weight
37.2 kDa
Background of SLAMF1
Signaling lymphocytic activation molecule 1 is a protein that in humans is encoded by the SLAMF1 gene. SLAMF1 Antibody: The signaling lymphocyte-activation molecule family member 1 (SLAMF1) is a novel receptor on T cells that potentiates T cell expansion in a CD28-independent manner. SLAMF1 is predominantly expressed by hematopoietic tissues. Reports suggest that the extracellular domain of SLAMF1 is the receptor for the measles virus and acts as a co-activator on both T and B cells. It is thought to interact with SH2D1A and with PTPN11 via its cytoplasmic domain. Mutations of the SLAM associated gene may be associated with X-linked lympho-proliferative disease (XLP).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03988-2 is guaranteed for ELISA, Flow Cytometry, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
IHC: human tonsil tissue
FCM: U937 cell
Validation Images & Assay Conditions
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IHC analysis of SLAM/CD150/SLAMF1 using anti-SLAM/CD150/SLAMF1 antibody (A03988-2).
SLAM/CD150/SLAMF1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLAM/CD150/SLAMF1 Antibody (A03988-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Flow Cytometry analysis of U937 cells using anti-SLAM/CD150/SLAMF1 antibody (A03988-2).
Overlay histogram showing U937 cells stained with A03988-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLAM/CD150/SLAMF1 Antibody (A03988-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-SLAM/CD150/SLAMF1 Antibody (A03988-2)
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