|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-SLC10A1/Ntcp Antibody|
|Description||Rabbit IgG polyclonal antibody for Sodium/bile acid cotransporter(SLC10A1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-SLC10A1/Ntcp Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1670)|
|Replacement Item||This antibody may replace the following items: sc-50893|sc-50892|sc-10921|sc-53903|sc-50891|sc-50890|sc-53860 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human SLC10A1(141-154aa YSRGIYDGDLKDKV), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Sodium/bile acid cotransporter|
|Molecular Weight||38119 MW|
|Protein Function||The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium.|
|Subcellular Localization||Membrane; Multi-pass membrane protein.|
|Alternative Names||Sodium/bile acid cotransporter;Cell growth-inhibiting gene 29 protein;Na(+)/bile acid cotransporter;Na(+)/taurocholate transport protein;Sodium/taurocholate cotransporting polypeptide;Solute carrier family 10 member 1;SLC10A1;NTCP;GIG29;|
|Research Areas|||signal transduction|metabolism|plasma membrane|channels| signal transduction|lipid metabolism| cancer|cancer metabolism|metabolic signaling pathway|metabolism of lipids and lipoproteins| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism||
Background for Sodium/bile acid cotransporter
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-SLC10A1/Ntcp Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-SLC10A1/Ntcp Antibody Images
Click the images to enlarge.
IHC(P): Human Liver Cancer Tissue
All lanes: Anti SLC10A1 (PA1670) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Predicted bind size: 38-56KD
Observed bind size: 49KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,