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- Table of Contents
SLC10A1 antibody (PA1670) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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IHC analysis of SLC10A1 using anti-SLC10A1 antibody (PA1670).
SLC10A1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC10A1 Antibody (PA1670) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
SLC10A1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC10A1 Antibody (PA1670) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Sodium/bile acid cotransporter SLC10A1 Antibody Picoband®
SLC10A1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
SLC10A1 antibody was positively detected by WB in:
human liver tissue, rat liver tissue, rat RH35 whole cell, rat kidney tissue, rat brain tissue, mouse liver tissue, mouse HEPA1-6 whole cell, mouse kidney tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SLC10A1 antibody was positively detected by IHC in:
mouse liver tissue, rat liver tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Sodium/bile acid cotransporter SLC10A1 Antibody Picoband®
View all SLC10A1 Antibodies
View all SLC10A1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SLC10A1, different from the related rat and mouse sequences by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated Molecular Weight
38.1 kDa
Background of SLC10A1
Na+-taurocholate cotransporting polypeptide (NTCP), also known as SLC10A1 (Solute carrier family 10, member 1), is the major bile acid uptake system in human hepatocytes. NTCP and the ileal transporter ASBT (apical sodium-dependent bile acid transporter) are two sodium-dependent transporters critical for the enterohepatic circulation of bile acids. The hASBT gene is known to be activated by the glucocorticoid receptor (GR). Ho RH et al. indicates functionally important polymorphisms in NTCP exist and that the like lihood of being carriers of such polymorphisms is dependent on ethnicity.
Cite This Product
Anti-Sodium/bile acid cotransporter SLC10A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1670)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
93%
High Homology
Rat
93%
High Homology
Cow
93%
High Homology
Sheep
93%
High Homology
Dog
93%
High Homology
Monkey
93%
High Homology
Zebrafish
43%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SLC10A1 antibody
Immunohistochemistry (IHC) protocol for SLC10A1 antibody
Publications
Application:
Species:
Farnesoid X Receptor: Effective alleviation of rifampicin -induced liver injury
Published: 2024-07-27
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 4.80
Species: Mouse
PMID: 39068755
Dimethyl fumarate attenuates cholestatic liver injury by activating the NRF2 and FXR pathways and suppressing NLRP3/GSDMD signaling in mice
Published: 2023-09-16
Journal: EXPERIMENTAL CELL RESEARCH
Application: WB
Impact Factor: 3.70
Species: Mouse
PMID: 37722551
Organic Anion Transporting Polypeptide (OATP) 1B3 is a Significant Transporter for Hepatic Uptake of Conjugated Bile Acids in Humans
Published: 2023-05-03
Journal: Cellular and Molecular Gastroenterology and Hepatology
Application: WB
Impact Factor: 7.20
Species: Mouse
PMID: 37146714
NRF2 and FXR dual signaling pathways cooperatively regulate the effects of oleanolic acid on cholestatic liver injury
Published: 2022-10-28
Journal: PHYTOMEDICINE
Application: IHC
Impact Factor: 6.66
Species: Rat
PMID: 36343550
Nrf2-Dependent Protective Effect of Paeoniflorin on α-Naphthalene Isothiocyanate-Induced Hepatic Injury
Published: 2022-06-22
Journal: AMERICAN JOURNAL OF CHINESE MEDICINE
Application: WB
Impact Factor: 6.01
Species: Mouse,Human
PMID: 35729506
Oleanolic acid alleviates ANIT-induced cholestatic liver injury by activating Fxr and Nrf2 pathways to ameliorate disordered bile acids homeostasis
Published: 2022-05-14
Journal: PHYTOMEDICINE
Application: IHC
Impact Factor: 6.66
Species: Rat
PMID: 35605478
Ergosterol peroxide inhibits HBV infection by inhibiting the binding of the pre-S1 domain of LHBsAg to NTCP
Published: 2021-10-07
Journal: ANTIVIRAL RESEARCH
Application: WB
Impact Factor: 5.97
Species: Human
PMID: 34627935
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Product Promise
We promise Anti-Sodium/bile acid cotransporter SLC10A1 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1670
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026