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Product Info Summary
| SKU: | A05138-4 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IP, WB |
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Product info
Product Name
Anti-ASBT antibody
SKU/Catalog Number
A05138-4
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-ASBT antibody catalog # A05138-4. Tested in WB, IP, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-ASBT antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05138-4)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human SLC10A2 recombinant protein (Position: S294-K348)
Reactive Species
A05138-4 is reactive to SLC10A2 in Human, Mouse
Observed Molecular Weight
38-40 kDa
Calculated molecular weight
37.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05138-4 is guaranteed for ELISA, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunoprecipitation, 1:50
ELISA, 1:100-1000
Positive Control
WB: human SH-SY5Y whole cell, human U251 whole cell, human Caco-2 whole cell, human PC-3 whole cell, mouse small intestine tissue
Validation Images & Assay Conditions
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Western blot analysis of ASBT using anti-ASBT antibody (A05138-4).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: mouse small intestine tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASBT antigen affinity purified polyclonal antibody (A05138-4) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ASBT at approximately 38-40 kDa. The expected band size for ASBT is at 38 kDa.
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