Anti-SLC12A1 Antibody

Product Name

Anti-SLC12A1 Antibody

View all NKCC2/SLC12A1 Antibodies

SKU/Catalog Number

PA2168

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SLC12A1 Antibody catalog # PA2168. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SLC12A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2168)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human SLC12A1, identical to the related mouse sequence, different from the related rat sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2168 is reactive to SLC12A1 in Human, Mouse, Rat

Applications

PA2168 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

121.45kDa

Background of NKCC2/SLC12A1

Solute carrier family 12 (sodium/potassium/chloride transporters), member 1, also called NKCC2 is specifically found in cells of the thick ascending limb of the loop of Henle in nephrons, the basic functional units of the kidney. This gene is mapped to 15q21.1. This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. This gene plays a vital role in the regulation of ionic balance and cell volume.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse

Validation Images & Assay Conditions

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Anti-SLC12A1 antibody, PA2168, IHC(F)
IHC(F): Rat Kidney Tissue

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Anti-SLC12A1 antibody, PA2168, Western blotting
WB: Rat Kidney Tissue Lysate

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Anti-SLC12A1 antibody, PA2168, IHC(P)
IHC(P): Rat Kidney Tissue

Gene/Protein Information For SLC12A1 (Source: Uniprot.org, NCBI)

Alternative Names

BSC1; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; Kidney-specific Na-K-Cl symporter; MGC48843; NKCC2; NKCC2A variant A; NKCC2Na-K-2Cl cotransporter; SLC12A1; solute carrier family 12 (sodium/potassium/chloride transporters), member 1; solute carrier family 12 member 1 SLC12A1 BSC1, NKCC2 solute carrier family 12 member 1 solute carrier family 12 member 1|NKCC2A variant A|Na-K-2Cl cotransporter|bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2|kidney-specific Na-K-Cl symporter|solute carrier family 12 (sodium/potassium/chloride transporter), member 1|solute carrier family 12 (sodium/potassium/chloride transporters), member 1

For more info on SLC12A1, check out the SLC12A1 Infographic

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In this infographic, you will see the following information for SLC12A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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