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- Table of Contents
SLC22A3 antibody (M04914) performs well in Flow Cytometry, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IHC analysis of SLC22A3 using anti-SLC22A3 antibody (M04914).
SLC22A3 was detected in a paraffin-embedded section of human cervical cance tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:400 rabbit anti-SLC22A3 Antibody (M04914) overnight at 4°C. Two-step IHC detection kit was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
SLC22A3 was detected in a paraffin-embedded section of human cervical cance tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:400 rabbit anti-SLC22A3 Antibody (M04914) overnight at 4°C. Two-step IHC detection kit was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-SLC22A3 Rabbit Monoclonal Antibody
SLC22A3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IHC, WB |
Tested Applications
SLC22A3 antibody was positively detected by WB in:
Human muscle cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-SLC22A3 Rabbit Monoclonal Antibody
View all SLC22A3 Antibodies
View all SLC22A3 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
25S19
Isotype
IgG
Immunogen
A synthesized peptide derived from human SLC22A3
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
75 kDa
Calculated Molecular Weight
61.3 kDa
Cite This Product
Anti-SLC22A3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04914)
Protocols
Western Blot protocol for SLC22A3 antibody
Publications
Application:
Species:
Unraveling the protective role of m6A methylation in SLC22A3 expression for breast Cancer intervention
Published: 2025-06-24
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
Application: IHC
Impact Factor: 4.20
Species: Human
PMID: 40570980
Review
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1 Review For Anti-SLC22A3 Rabbit Monoclonal Antibody
In this IHC experiment using Anti-SLC22A3 antibody (Cat# M04914), strong and clear expression was observed in cervical cancer tumor cells, with further comparison planned between tumor and adjacent tissues.
| SKU | M04067-2 |
|---|---|
| Application | Immunohistochemistry |
| Sample | human cervical cancer tissue |
| Sample Processing Description | Cervical cancer tissue collected from clinical surgery, fixed in formalin and paraffin-embedded |
| Other Reagents | Goat serum, DAB substrate solution |
| Primary Antibody | SLC22A3 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:200, overnight at 4 ℃ |
| Secondary Antibody | Two-step IHC detection kit |
| Secondary Incubation | 30 min at 37℃ |
| Detection | Imaging system:Leica DM2500 |
| Results Summary | IHC using SLC22A3 antibody (M04914) showed strong and clear expression in tumor cells of human cervical carcinoma, and the next step will be to compare expression between tumor and adjacent normal tissue. |
Juan Wang, The First Affiliated Hospital of Shihezi University
Verified customer
Submitted 2026-02-25
Product Promise
We promise Anti-SLC22A3 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M04914
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026