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Product Info Summary
| SKU: | A14683-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SLC26A10P Antibody Picoband®
SKU/Catalog Number
A14683-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SLC26A10P Antibody Picoband® catalog # A14683-1. Tested in WB, IHC applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SLC26A10P Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14683-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SLC26A10P.
Reactive Species
A14683-1 is reactive to SLC26A10P in Human
Observed Molecular Weight
60 kDa
Calculated molecular weight
60.1 kDa
Background of SLC26A10P
Predicted to enable inorganic anion transmembrane transporter activity and organic anion transmembrane transporter activity. Predicted to be involved in chloride transmembrane transport and sulfate transmembrane transport. Predicted to be located in membrane. Predicted to be active in plasma membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14683-1 is guaranteed for IHC, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Human |
| Immunohistochemistry(Paraffin-embedded Section) | 2-5 μg/ml | Human |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Validation Images & Assay Conditions
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Western blot analysis of SLC26A10 using anti-SLC26A10 antibody (A14683-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HGC-27 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC26A10 antigen affinity purified polyclonal antibody (A14683-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC26A10 at approximately 60 kDa. The expected band size for SLC26A10 is at 60 kDa.
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IHC analysis of SLC26A10 using anti-SLC26A10 antibody (A14683-1).
SLC26A10 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC26A10 Antibody (A14683-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-SLC26A10P Antibody Picoband® (A14683-1)
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