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Product Info Summary
| SKU: | A07581-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-SLC26A9 Antibody Picoband®
SKU/Catalog Number
A07581-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-SLC26A9 Antibody Picoband® catalog # A07581-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SLC26A9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07581-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human SLC26A9 recombinant protein (Position: K27-D763). Human SLC26A9 shares 89.7% amino acid (aa) sequence identity with mouse SLC26A9.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A07581-1 is reactive to SLC26A9 in Human, Mouse, Rat
Observed Molecular Weight
90 kDa
Calculated molecular weight
87.0 kDa
Background of SLC26A9
The members of the SLC26 family of transporters, such as SLC26A9, are well conserved across species and mediate the electroneutral exchange of chloride for bicarbonate or sulfate across the plasma membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07581-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HepG2 whole cell, rat stomach tissue, rat lung tissue, rat C6 whole cell, mouse stomach tissue, mouse lung tissue, mouse MFC whole cell
FCM: HepG2 cell
Validation Images & Assay Conditions
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Western blot analysis of SLC26A9 using anti-SLC26A9 antibody (A07581-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: rat stomach tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse stomach tissue lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse MFC whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC26A9 antigen affinity purified polyclonal antibody (Catalog # A07581-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC26A9 at approximately 90 kDa. The expected band size for SLC26A9 is at 87 kDa.
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Flow Cytometry analysis of HepG2 cells using anti-SLC26A9 antibody (A07581-1).
Overlay histogram showing HepG2 cells stained with A07581-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC26A9 Antibody (A07581-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (A07581-1, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Specific Publications For Anti-SLC26A9 Antibody Picoband® (A07581-1)
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