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Product Info Summary
| SKU: | A12449 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-SLC35D2 Antibody
SKU/Catalog Number
A12449
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SLC35D2 Antibody (Catalog # A12449). Tested in ELISA, WB, ICC applications. This antibody reacts with Human.
Storage & Handling
SLC35D2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SLC35D2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12449)
Host
Rabbit
Contents
SLC35D2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SLC35D2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human SLC35D2. The immunogen is located within the last 50 amino acids of SLC35D2.
Cross-reactivity
At least two isoforms of SLC35D2 are known to exist; this antibody will recognize both isoforms. SLC35D2 antibody is predicted to not cross-react with SLC35D1 or SLC35D3.
Reactive Species
A12449 is reactive to SLC35D2 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
36.7 kDa
Background of SLC35D2
The solute carrier family SLC35 consists of at least 17 proteins that act as nucleotide sugar transporters localized to the Golgi apparatus and endoplasmic reticulum. The role of the ER-resident SLC family member SLC35D2 is to transport both UDP-glucuronic acid and UDP-N-acetylgalactosamine. Its overexpression in transfected cells modulated cell surface heparin sulfate expression, suggesting that SLC35D2 is involved in heparin sulfate synthesis. SLC35D2-overexpressing cells also showed increased constitutive and hypotonic stress-stimulated release of UDP-GlcNAc, suggesting that SLC35D2 may be involved in UDP-sugar release and cell signaling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12449 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SLC35D2 antibody can be used for detection of SLC35D2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SLC35D2 in HeLa cell lysate with SLC35D2 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of SLC35D2 in HeLa cells with SLC35D2 antibody at 5 μg/mL.
Specific Publications For Anti-SLC35D2 Antibody (A12449)
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