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- Table of Contents
SLC7A11 antibody (A03036-2) performs well in ELISA, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 13 Publications
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IHC analysis of xCT/SLC7A11 using anti-xCT/SLC7A11 antibody (A03036-2).
xCT/SLC7A11 was detected in a paraffin-embedded section of human ovraian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-xCT/SLC7A11 Antibody (A03036-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
xCT/SLC7A11 was detected in a paraffin-embedded section of human ovraian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-xCT/SLC7A11 Antibody (A03036-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-xCT/SLC7A11 Antibody Picoband®
SLC7A11 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IHC, WB |
Tested Applications
SLC7A11 antibody was positively detected by WB in:
human HepG2 whole cell, human A549 whole cell, human Hela whole cell, human U2OS whole cell, rat C6 whole cell, mouse Neuro-2a whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ELISA
Human
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Product Information
Product Name
Anti-xCT/SLC7A11 Antibody Picoband®
View all SLC7A11 Antibodies
View all SLC7A11 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
Peptide
Cross-Reactivity
No cross reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40-50 kDa
Calculated Molecular Weight
55.4 kDa
Background of SLC7A11
This gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. This protein has been identified as the predominant mediator of Kaposi sarcoma-associated herpesvirus fusion and entry permissiveness into cells. Also, increased expression of this gene in primary gliomas (compared to normal brain tissue) was associated with increased glutamate secretion via the XCT channels, resulting in neuronal cell death.
Cite This Product
Anti-xCT/SLC7A11 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03036-2)
Protocols
Western Blot protocol for SLC7A11 antibody
Publications
Application:
Species:
EP300 drives the progression of oral squamous cell carcinoma by regulating NUPR1-mediated ferroptosis and EMT
Published: 2026-08-26
Journal: CLINICAL AND EXPERIMENTAL MEDICINE
Application: WB
Impact Factor: 4.50
Species: Human,Mouse
Polygonatum odoratum extract ameliorates acetaminophen-induced acute liver injury by modulating the gut-liver axis and remodeling microbial and ceramide metabolism to suppress hepatic ferroptosis: insights from multi-omics
Published: 2026-07-29
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 5.40
Species: Mouse
PMID: 42591220
Scutellaria Barbata Water Extract Suppresses Breast Cancer Growth and Lung Metastasis by Inducing Ferroptosis
Published: 2026-06-04
Journal: INTEGRATIVE CANCER THERAPIES
Application: WB
Impact Factor: 3.10
Species: Mouse,Human
PMID: 42244109
Myricetin inhibits vascular calcification in an in vitro model by modulating ferroptosis-related SLC7A11/GPX4 signaling
Published: 2026-04-01
Journal: JOURNAL OF PHARMACOLOGICAL SCIENCES
Application: WB
Impact Factor: 2.90
Species: Rat
PMID: 42025374
A Biomimetic Nanoagonist for Synergistic Photodynamic/Ferroptosis/Disulfidptosis Therapy via Coinhibition of Glucose Metabolism and the SLC7A11/GSH/GPX4 Axis
Published: 2026-03-04
Journal: ACS Applied Materials & Interfaces
Application: WB
Impact Factor: 8.20
Species: Mouse,Human
PMID: 41779630
Yishen Gushu formula exerts osteoprotective effects in OVX-Induced PMOP rats: role of ferroptosis Inhibition and iron metabolism correction
Published: 2026-02-04
Journal: HEREDITAS
Application: WB,IHC
Impact Factor: 2.50
Species: Rat
PMID: 41639935
DNMT3A-dependent suppression of HIF-1α alleviates myopia by reducing scleral ferroptosis and fibroblast extracellular matrix remodeling
Published: 2025-12-15
Journal: AMERICAN JOURNAL OF PATHOLOGY
Application: IHC
Impact Factor: 3.60
Species: Rat
PMID: 41407196
Integrated bioinformatics analysis identifies glutathione metabolism-related genes as diagnostic biomarkers for periodontitis
Published: 2025-10-06
Journal: BMC Oral Health
Application: IHC
Impact Factor: 3.10
Species: Rat
PMID: 41053677
Diselenide Nanogels Modulate Mitochondrial Function and Mitigate Oxidative Stress in Cardiomyocytes for Enhanced Cardiac Repair
Published: 2025-03-03
Journal: ACS Applied Materials & Interfaces
Application: IF
Impact Factor: 8.20
Species: Rat
PMID: 40028900
CircTTC13 promotes sorafenib resistance in hepatocellular carcinoma through the inhibition of ferroptosis by targeting the miR-513a-5p/SLC7A11 axis
Published: 2025-01-27
Journal: Molecular Cancer
Application: WB
Impact Factor: 33.90
Species: Human,Mouse
PMID: 39871338
Bergapten Ameliorates Renal Fibrosis by Inhibiting Ferroptosis
Published: 2025-01-07
Journal: PHYTOTHERAPY RESEARCH
Application: WB
Impact Factor: 6.30
Species: Human
PMID: 39764683
An in-depth analysis of the effects of excessive acetochlor exposure on chicken liver health
Published: 2024-12-27
Journal: PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
Application: WB
Impact Factor: 4.20
Species: Chicken
PMID: 40015872
Porphyromonas gingivalis as a promotor in the development of the alcoholic liver disease via ferroptosis
Published: 2023-11-13
Journal: MICROBES AND INFECTION
Application: IHC,WB
Impact Factor: 5.80
Species: Mouse,Human
PMID: 37967609
Review
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1 Review For Anti-xCT/SLC7A11 Antibody Picoband®
The SLC7A11 antibody was used to detect the expression of the target protein in human uterine tissue. Although two bands were observed in the experiment, this did not affect the interpretation of the results.
| SKU | A03036-2 |
|---|---|
| Application | Western Blot |
| Sample | human uterine tissue |
| Sample Processing Description | The tissue was minced and further disrupted by sonication, then lysed on ice for 1 hour using RIPA buffer. After centrifugation, the supernatant was collected and quantified using the BCA method. The appropriate amount of loading buffer was added, and the samples were boiled in a water bath to denature the proteins. Finally, 15 μL of each protein sample was loaded into each lane of the SDS-PAGE gel. |
| Other Reagents | 5% Non-fat milk |
| Primary Antibody | Anti-xCT/SLC7A11 Antibody Picoband® |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | 1 hour in room temperature |
| Detection | Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:Tanon |
| Results Summary | The SLC7A11 antibody was used to detect the expression of the target protein in human uterine tissue. Although two bands were observed in the experiment, this did not affect the interpretation of the results. |
Anfeng Ning, Peking University Third Hospital
Verified customer
Submitted 2025-11-11
Product Promise
We promise Anti-xCT/SLC7A11 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A03036-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026