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Product Info Summary
| SKU: | A17269 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SLFN13 Antibody Picoband®
SKU/Catalog Number
A17269
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-SLFN13 Antibody Picoband® catalog # A17269. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SLFN13 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17269)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human SLFN13 recombinant protein (Position: Q62-R797). Human SLFN13 shares 62.1% amino acid (aa) sequence identity with rat SLFN13.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A17269 is reactive to SLFN13 in Human, Mouse, Rat
Observed Molecular Weight
102 kDa
Calculated molecular weight
102.0 kDa
Background of SLFN13
Schlafen family members are preferentially expressed in lymphoid tissues and are differentially regulated during thymocyte maturation. Schlafen proteins function as suppressors of cell growth and are thought to play a role in the maintenance of T cell quiescence. All members of the Schlafen family contain a conserved core domain and are substantially diversified at the N terminus. Changes in Schalfen protein expression may contribute to phenotypic differences seen in thymic subsets. Slfn13 (Schlafen family member 13), also known as SLFN10, is an 897 amino acid protein that exists as 2 alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 17q12.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A17269 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of SLFN13 using anti-SLFN13 antibody (A17269).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human Hacat whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse C2C12 whole cell lysates,
Lane 8: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLFN13 antigen affinity purified polyclonal antibody (A17269) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLFN13 at approximately 102 kDa. The expected band size for SLFN13 is at 102 kDa.
Specific Publications For Anti-SLFN13 Antibody Picoband® (A17269)
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