Anti-SLMAP Antibody Picoband™

Product Name

Anti-SLMAP Antibody Picoband™

View all SLMAP Antibodies

SKU/Catalog Number

A07262

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SLMAP Antibody Picoband™ catalog # A07262. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SLMAP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07262)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SLMAP recombinant protein (Position: D25-E607).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07262 is reactive to SLMAP in Human, Mouse

Applications

A07262 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

95 kDa

Calculated molecular weight

95.198kDa

Background of SLMAP

Sarcolemmal membrane-associated protein is a protein that in humans is encoded by the SLMAP gene. This gene encodes a component of a conserved striatin-interacting phosphatase and kinase complex. Striatin family complexes participate in a variety of cellular processes including signaling, cell cycle control, cell migration, Golgi assembly, and apoptosis. The protein encoded by this gene is a coiled-coil, tail-anchored membrane protein with a single C-terminal transmembrane domain that is posttranslationally inserted into membranes. Mutations in this gene are associated with Brugada syndrome, a cardiac channelopathy. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SLMAP using anti-SLMAP antibody (A07262).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human SIHA whole cell lysates,
Lane 5: mouse heart tissue lysates,
Lane 6: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLMAP antigen affinity purified polyclonal antibody (Catalog # A07262) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLMAP at approximately 95 kDa. The expected band size for SLMAP is at 95 kDa.

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Figure 2. Flow Cytometry analysis of K562 cells using anti-SLMAP antibody (A07262).
Overlay histogram showing K562 cells stained with A07262 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLMAP Antibody (A07262, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SLMAP (Source: Uniprot.org, NCBI)

Alternative Names

FLJ42206; KIAA1601Sarcolemmal-associated protein; MGC138760; sarcolemma associated protein; sarcolemmal membrane-associated protein; SLAPMGC138761 SLMAP SLAP sarcolemma associated protein sarcolemmal membrane-associated protein

For more info on SLMAP, check out the SLMAP Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SLMAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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