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- Table of Contents
SMAD1 antibody (M00728-1) performs well in IF, ICC, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Anti-Smad1 Rabbit Monoclonal Antibody
SMAD1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | IF, ICC, WB |
Tested Applications
SMAD1 antibody was positively detected by WB in:
human Hela whole cell, human Jurkat whole cell, human SIHA whole cell, human HepG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SMAD1 antibody was positively detected by IHC in:
human kidney tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
Human
Tested
Tested
Recommend
Recommend
Mouse
Recommend
Recommend
Product Information
Product Name
Anti-Smad1 Rabbit Monoclonal Antibody
View all SMAD1 Antibodies
View all SMAD1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CAC-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Smad1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
52 kDa
Calculated Molecular Weight
52.3 kDa
Cite This Product
Anti-Smad1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00728-1)
Protocols
Western Blot protocol for SMAD1 antibody
Immunohistochemistry (IHC) protocol for SMAD1 antibody
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Product Promise
We promise Anti-Smad1 Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00728-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026