Anti-SMAD1/SMAD5 Antibody Picoband™

SMAD1 antibody

Boster Bio Anti-SMAD1/SMAD5 Antibody Picoband™ catalog # A00728-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00728-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-SMAD1/SMAD5 Antibody Picoband™

View all SMAD1 Antibodies

SKU/Catalog Number

A00728-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SMAD1/SMAD5 Antibody Picoband™ catalog # A00728-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SMAD1/SMAD5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00728-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human SMAD1/SMAD5, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00728-1 is reactive to SMAD1 in Human, Mouse, Rat

Applications

A00728-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa, 60 kDa

Calculated molecular weight

52.26kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For SMAD1 (Source: Uniprot.org, NCBI)

Gene Name

SMAD1

Full Name

Mothers against decapentaplegic homolog 1

Weight

52.26kDa

Superfamily

dwarfin/SMAD family

Alternative Names

BSP1; BSP-1; hSMAD1; JV4-1SMAD, mothers against DPP homolog 1 (Drosophila); MAD homolog 1; MADH1JV41; MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila); Mad-related protein 1; mothers against decapentaplegic homolog 1; Mothers against DPP homolog 1; SMAD family member 1SMAD 1; SMAD, mothers against DPP homolog 1; Smad1; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1 SMAD1 BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1 SMAD family member 1 mothers against decapentaplegic homolog 1|MAD, mothers against decapentaplegic homolog 1|Mad-related protein 1|SMAD, mothers against DPP homolog 1|TGF-beta signaling protein 1|mothers against DPP homolog 1|transforming growth factor-beta signaling protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SMAD1, check out the SMAD1 Infographic

SMAD1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SMAD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-SMAD1/SMAD5 Antibody Picoband™

Question

We are currently using anti-SMAD1/SMAD5 antibody A00728-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-02

Answer

The anti-SMAD1/SMAD5 antibody (A00728-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-02

Question

We have seen staining in rat visceral pleura. What should we do? Is anti-SMAD1/SMAD5 antibody supposed to stain visceral pleura positively?

Verified Customer

Verified customer

Asked: 2019-12-18

Answer

According to literature visceral pleura does express SMAD1. According to Uniprot.org, SMAD1 is expressed in visceral pleura, kidney, mammary carcinoma, placenta, uterus, ovarian carcinoma, among other tissues. Regarding which tissues have SMAD1 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 8637600
Mammary carcinoma, Pubmed ID: 8663601
Ovarian carcinoma, Pubmed ID: 9136927
Placenta, Pubmed ID: 8774881, 15489334
Uterus, Pubmed ID: 14702039, 17974005

Boster Scientific Support

Answered: 2019-12-18

Question

My team were satisfied with the WB result of your anti-SMAD1/SMAD5 antibody. However we have been able to see positive staining in visceral pleura cytoplasm. using this antibody. Is that expected? Could you tell me where is SMAD1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-11-22

Answer

Based on literature, visceral pleura does express SMAD1. Generally SMAD1 expresses in cytoplasm. Regarding which tissues have SMAD1 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 8637600
Mammary carcinoma, Pubmed ID: 8663601
Ovarian carcinoma, Pubmed ID: 9136927
Placenta, Pubmed ID: 8774881, 15489334
Uterus, Pubmed ID: 14702039, 17974005

Boster Scientific Support

Answered: 2019-11-22

Question

We need using your anti-SMAD1/SMAD5 antibody for positive regulation of transcription by rna polymerase ii studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

C. Banerjee

Verified customer

Asked: 2013-11-13

Answer

We appreciate your inquiry. This A00728-1 anti-SMAD1/SMAD5 antibody is validated on rat kidney tissue, tissue lysate, testis tissue, mouse kidney tissue, brain tissue, human hela, hela cell lysate, hepg2 cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2013-11-13

Order DetailsPrice
A00728-1

100μg

$370
A00728-1-10ug

10μg sample (liquid)

$99
A00728-1-Biotin

100 μg Biotin conjugated

$570
A00728-1-Cy3

100 μg Cy3 conjugated

$570
A00728-1-Dylight488

100 μg Dylight488 conjugated

$570
A00728-1-Dylight550

100 μg Dylight550 conjugated

$570
A00728-1-Dylight594

100 μg Dylight594 conjugated

$570
A00728-1-FITC

100 μg FITC conjugated

$570
A00728-1-HRP

100 μg HRP conjugated

$570
A00728-1-APC

100 μg APC conjugated

$670
A00728-1-PE

100 μg PE conjugated

$670
A00728-1-carrier-free

Carrier Free

$370

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A00728-1
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