Product Info Summary
SKU: | A00728-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-SMAD1/SMAD5 Antibody Picoband™
SKU/Catalog Number
A00728-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SMAD1/SMAD5 Antibody Picoband™ catalog # A00728-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SMAD1/SMAD5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00728-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SMAD1/SMAD5, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00728-1 is reactive to SMAD1 in Human, Mouse, Rat
Applications
A00728-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
52 kDa, 60 kDa
Calculated molecular weight
52.26kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SMAD1 using anti-SMAD1 antibody (A00728-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysate,
Lane 2: rat testis tissue lysate,
Lane 3: mouse brain tissue lysate,
Lane 4: mouse kidney tissue lysate,
Lane 5: mouse testis tissue lysate,
Lane 6: human Hela cell lysate,
Lane 7: human A375 cell lysate,
Lane 8: human HepG2 cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD1 antigen affinity purified polyclonal antibody (Catalog # A00728-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD1 at approximately 52,60KD. The expected band size for SMAD1 is at 48, 52, 60KD.
Protein Target Info & Infographic
Gene/Protein Information For SMAD1 (Source: Uniprot.org, NCBI)
Gene Name
SMAD1
Full Name
Mothers against decapentaplegic homolog 1
Weight
52.26kDa
Superfamily
dwarfin/SMAD family
Alternative Names
BSP1; BSP-1; hSMAD1; JV4-1SMAD, mothers against DPP homolog 1 (Drosophila); MAD homolog 1; MADH1JV41; MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila); Mad-related protein 1; mothers against decapentaplegic homolog 1; Mothers against DPP homolog 1; SMAD family member 1SMAD 1; SMAD, mothers against DPP homolog 1; Smad1; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1 SMAD1 BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1 SMAD family member 1 mothers against decapentaplegic homolog 1|MAD, mothers against decapentaplegic homolog 1|Mad-related protein 1|SMAD, mothers against DPP homolog 1|TGF-beta signaling protein 1|mothers against DPP homolog 1|transforming growth factor-beta signaling protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMAD1, check out the SMAD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMAD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SMAD1/SMAD5 Antibody Picoband™ (A00728-1)
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No publications found for A00728-1
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-SMAD1/SMAD5 Antibody Picoband™
Question
We are currently using anti-SMAD1/SMAD5 antibody A00728-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
The anti-SMAD1/SMAD5 antibody (A00728-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-02
Question
We have seen staining in rat visceral pleura. What should we do? Is anti-SMAD1/SMAD5 antibody supposed to stain visceral pleura positively?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
According to literature visceral pleura does express SMAD1. According to Uniprot.org, SMAD1 is expressed in visceral pleura, kidney, mammary carcinoma, placenta, uterus, ovarian carcinoma, among other tissues. Regarding which tissues have SMAD1 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 8637600
Mammary carcinoma, Pubmed ID: 8663601
Ovarian carcinoma, Pubmed ID: 9136927
Placenta, Pubmed ID: 8774881, 15489334
Uterus, Pubmed ID: 14702039, 17974005
Boster Scientific Support
Answered: 2019-12-18
Question
My team were satisfied with the WB result of your anti-SMAD1/SMAD5 antibody. However we have been able to see positive staining in visceral pleura cytoplasm. using this antibody. Is that expected? Could you tell me where is SMAD1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
Based on literature, visceral pleura does express SMAD1. Generally SMAD1 expresses in cytoplasm. Regarding which tissues have SMAD1 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 8637600
Mammary carcinoma, Pubmed ID: 8663601
Ovarian carcinoma, Pubmed ID: 9136927
Placenta, Pubmed ID: 8774881, 15489334
Uterus, Pubmed ID: 14702039, 17974005
Boster Scientific Support
Answered: 2019-11-22
Question
We need using your anti-SMAD1/SMAD5 antibody for positive regulation of transcription by rna polymerase ii studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
C. Banerjee
Verified customer
Asked: 2013-11-13
Answer
We appreciate your inquiry. This A00728-1 anti-SMAD1/SMAD5 antibody is validated on rat kidney tissue, tissue lysate, testis tissue, mouse kidney tissue, brain tissue, human hela, hela cell lysate, hepg2 cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-11-13