Anti-Smad6 Monoclonal Antibody

Product Name

Anti-Smad6 Monoclonal Antibody

View all SMAD6 Antibodies

SKU/Catalog Number

M02227

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Smad6 Monoclonal Antibody catalog # M02227. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Smad6 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02227)

Host

Mouse

Contents

Ascitic fluid containing 0.03% sodium azide.

Clonality

Monoclonal

Clone Number

5H3

Isotype

IgG

Immunogen

Purified recombinant fragment of human Smad6 expressed in E. Coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M02227 is reactive to SMAD6 in Human

Applications

M02227 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

53.497kDa

Background of SMAD6

LGR4, also known as leucine-rich repeat-containing G protein-coupled receptor 4, is a G protein-coupled receptors (GPCRs). GPCRs are membrane bound proteins that play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain. LGR4 is an orphan GPCR reported to be expressed in steroidogenic tissues such as placenta, ovary, testis, adrenal, pancreas, prostate, and thyroid, as well as in spinal cord, stomach, heart, and kidney.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ELISA 1:10000

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-Smad6 Monoclonal Antibody M02227

Western Blot (WB) analysis using Smad6 Monoclonal Antibody against A431 (1)

Gene/Protein Information For SMAD6 (Source: Uniprot.org, NCBI)

Alternative Names

hSMAD6; HsT17432; MAD homolog 6; MADH6SMAD, mothers against DPP homolog 6 (Drosophila); MADH7; mothers against decapentaplegic homolog 6; Mothers against decapentaplegic, drosophila, homolog of, 6; Mothers against DPP homolog 6; SMAD 6; SMAD family member 6MAD, mothers against decapentaplegic homolog 6 (Drosophila); SMAD, mothers against DPP homolog 6; Smad6 SMAD6 AOVD2, HsT17432, MADH6, MADH7 SMAD family member 6 mothers against decapentaplegic homolog 6|MAD homolog 6|SMAD, mothers against DPP homolog 6|mothers against decapentaplegic, drosophila, homolog of, 6

For more info on SMAD6, check out the SMAD6 Infographic

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In this infographic, you will see the following information for SMAD6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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