Anti-SmarcAL1 Antibody

Product Name

Anti-SmarcAL1 Antibody

View all SMARCAL1 Antibodies

SKU/Catalog Number

A03383

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-SmarcAL1 Antibody (Catalog # A03383). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-SmarcAL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03383)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal near aa 75-100 of human SmarcAL1 protein.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A03383 is reactive to SMARCAL1 in Human

Calculated molecular weight

105.9 kDa

Background of SMARCAL1

SmarcAL1 (SWI/SNF-related µatrix-associated actin-dependent regulator of chromatin a-like 1) is also known as HARP, HepA Related Protein, HHARP, SMARCA like Protein 1 and SWI/SNF Related. SmarcAL1 is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03383 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:16,000 - 1:64,000
WB: 1:500 - 1:3,000
This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 106 kDa in size corresponding to SmarcAL1 by western blotting in the appropriate cell lysate or extract.

Validation Images & Assay Conditions

See full target information SMARCAL1

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