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Product Info Summary
| SKU: | A07318-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SMARCD1 Antibody Picoband®
SKU/Catalog Number
A07318-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SMARCD1 Antibody Picoband® catalog # A07318-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SMARCD1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07318-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SMARCD, identical to the related mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07318-1 is reactive to SMARCD1 in Human
Observed Molecular Weight
58 kDa
Calculated molecular weight
58.2 kDa
Background of SMARCD1
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 is a protein that in humans is encoded by the SMARCD1 gene. The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07318-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Positive Control
WB: human Hela whole cell, human 293T whole cell, human PANC-1 whole cell, human Jurkat whole cell, human MOLT-4 whole cell, human Caco-2 whole cell, human SH-SY5Y whole cell, human Daudi whole cell
Validation Images & Assay Conditions
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Western blot analysis of SMARCD1 using anti-SMARCD1 antibody (A07318-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human PANC-1 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: human MOLT-4 whole cell lysates,
Lane 6: human Caco-2 whole cell lysates,
Lane 7: human SH-SY5Y whole cell lysates,
Lane 8: human Daudi whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCD1 antigen affinity purified polyclonal antibody (Catalog # A07318-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMARCD1 at approximately 58 kDa. The expected band size for SMARCD1 is at 58 kDa.
Specific Publications For Anti-SMARCD1 Antibody Picoband® (A07318-1)
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