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Product Info Summary
| SKU: | A10280-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-SMC1B Antibody Picoband®
SKU/Catalog Number
A10280-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-SMC1B Antibody Picoband® catalog # A00601-1. Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SMC1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10280-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human SMC1B recombinant protein (Position: R106-D1221). Human SMC1B shares 83.2% amino acid (aa) sequence identity with mouse SMC1B.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A10280-1 is reactive to SMC1B in Human, Mouse, Rat
Observed Molecular Weight
150,250 kDa
Calculated molecular weight
143.9 kDa
Background of SMC1B
Structural maintenance of chromosomes protein 1B (SMC-1B) is a protein that in humans is encoded by the SMC1B gene. SMC1L2 belongs to a family of proteins required for chromatid cohesion and DNA recombination during meiosis and mitosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10280-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat testis tissue, rat C6 whole cell, mouse testis tissue, mouse Neuro-2a whole cell
ICC/IF: U2OA cell
Validation Images & Assay Conditions
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Western blot analysis of SMC1B using anti-SMC1B antibody (A10280-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: rat C6 whole cell lysates,
Lane 3: mouse testis tissue lysates,
Lane 4: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC1B antigen affinity purified polyclonal antibody (Catalog # A10280-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC1B at approximately 150,250 kDa. The expected band size for SMC1B is at 144 kDa.
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IF analysis of SMC1B using anti-SMC1B antibody (A10280-1).
SMC1B was detected in an immunocytochemical section of U2OA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SMC1B Antibody (A10280-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-SMC1B Antibody Picoband® (A10280-1)
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