Anti-SMF HMGXB3 Antibody

HMGXB3 antibody

Boster Bio Anti-SMF HMGXB3 Antibody (Catalog# A16486). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat, Mouse.

Product Info Summary

SKU: A16486
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC-P, IF (paraffin)

Product Name

Anti-SMF HMGXB3 Antibody

View all HMGXB3 Antibodies

SKU/Catalog Number

A16486

Size

100ul

Form

Liquid

Description

Boster Bio Anti-SMF HMGXB3 Antibody (Catalog# A16486). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SMF HMGXB3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16486)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human HMGXB3. Immunogen sequence location: 131-180

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A16486 is reactive to HMGXB3 in Human, Mouse, Rat

Applications

A16486 is guaranteed for ELISA, IHC-P, IF (paraffin) Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

168.334kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For HMGXB3 (Source: Uniprot.org, NCBI)

Gene Name

HMGXB3

Full Name

HMG domain-containing protein 3

Weight

168.334kDa

Alternative Names

HMG domain-containing protein 3 HMGXB3 HMGX3, SMF HMG-box containing 3 HMG domain-containing protein 3|HMG box domain containing 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HMGXB3, check out the HMGXB3 Infographic

HMGXB3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HMGXB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-SMF HMGXB3 Antibody

Question

Is this A16486 anti-SMF antibody reactive to the isotypes of HMGXB3?

Verified Customer

Verified customer

Asked: 2020-03-27

Answer

The immunogen of A16486 anti-SMF antibody is A peptide derived from human HMGXB3. Immunogen sequence location: 131-180. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-03-27

Question

I have attached the WB image, lot number and protocol we used for olfactory bulb using anti-SMF antibody A16486. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-26

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-26

Question

Would anti-SMF antibody A16486 work on pig IHC with placenta?

Verified Customer

Verified customer

Asked: 2019-11-21

Answer

Our lab technicians have not tested anti-SMF antibody A16486 on pig. You can run a BLAST between pig and the immunogen sequence of anti-SMF antibody A16486 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig placenta in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-21

Question

I am interested in to test anti-SMF antibody A16486 on human olfactory bulb for research purposes, then I may be interested in using anti-SMF antibody A16486 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

The products we sell, including anti-SMF antibody A16486, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-09

Question

We are currently using anti-SMF antibody A16486 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2018-02-13

Answer

The anti-SMF antibody (A16486) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-02-13

Question

Would anti-SMF antibody A16486 work for IHC with olfactory bulb?

Verified Customer

Verified customer

Asked: 2017-07-27

Answer

According to the expression profile of olfactory bulb, HMGXB3 is highly expressed in olfactory bulb. So, it is likely that anti-SMF antibody A16486 will work for IHC with olfactory bulb.

Boster Scientific Support

Answered: 2017-07-27

Question

Is a blocking peptide available for product anti-SMF antibody (A16486)?

C. Krishna

Verified customer

Asked: 2016-05-17

Answer

We do provide the blocking peptide for product anti-SMF antibody (A16486). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-05-17

Order DetailsPrice
A16486

100 μL

$335

Free Secondary Antibody

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A16486
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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