Click image to see more details
Product Info Summary
| SKU: | A16486 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-SMF HMGXB3 Antibody
SKU/Catalog Number
A16486
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SMF HMGXB3 Antibody (Catalog# A16486). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SMF HMGXB3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16486)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human HMGXB3. Immunogen sequence location: 131-180
Reactive Species
A16486 is reactive to HMGXB3 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
168.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16486 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SMF Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-SMF HMGXB3 Antibody (A16486)
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Customer Q&As
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7 Customer Q&As for Anti-SMF HMGXB3 Antibody
Question
Is this A16486 anti-SMF antibody reactive to the isotypes of HMGXB3?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
The immunogen of A16486 anti-SMF antibody is A peptide derived from human HMGXB3. Immunogen sequence location: 131-180. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-27
Question
I have attached the WB image, lot number and protocol we used for olfactory bulb using anti-SMF antibody A16486. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-26
Question
Would anti-SMF antibody A16486 work on pig IHC with placenta?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
Our lab technicians have not tested anti-SMF antibody A16486 on pig. You can run a BLAST between pig and the immunogen sequence of anti-SMF antibody A16486 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig placenta in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-21
Question
I am interested in to test anti-SMF antibody A16486 on human olfactory bulb for research purposes, then I may be interested in using anti-SMF antibody A16486 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
The products we sell, including anti-SMF antibody A16486, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-09
Question
We are currently using anti-SMF antibody A16486 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-13
Answer
The anti-SMF antibody (A16486) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-13
Question
Would anti-SMF antibody A16486 work for IHC with olfactory bulb?
Verified Customer
Verified customer
Asked: 2017-07-27
Answer
According to the expression profile of olfactory bulb, HMGXB3 is highly expressed in olfactory bulb. So, it is likely that anti-SMF antibody A16486 will work for IHC with olfactory bulb.
Boster Scientific Support
Answered: 2017-07-27
Question
Is a blocking peptide available for product anti-SMF antibody (A16486)?
C. Krishna
Verified customer
Asked: 2016-05-17
Answer
We do provide the blocking peptide for product anti-SMF antibody (A16486). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2016-05-17
