Anti-Protein SMG7 SMG7 Antibody

Product Name

Anti-Protein SMG7 SMG7 Antibody

View all SMG7 Antibodies

SKU/Catalog Number

A06078

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Protein SMG7 SMG7 Antibody (Catalog # A06078). Tested in WB, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Protein SMG7 SMG7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06078)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

Synthesized peptide derived from internal of human SMG7.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06078 is reactive to SMG7 in Human

Applications

A06078 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

127.282kDa

Background of SMG7

Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation.

Ohnishi T., Mol. Cell 12:1187-1200(2003).
Nagase T., DNA Res. 3:321-329(1996).
Nakajima D., DNA Res. 9:99-106(2002).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

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Western blot analysis of extracts from HeLa cells, Jurkat cells, 293 cells and HUVEC cells, using SMG7 antibody A06078.

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Immunofluorescence analysis of HepG2 cells, using SMG7 antibody A06078.

Gene/Protein Information For SMG7 (Source: Uniprot.org, NCBI)

Alternative Names

breast cancer-associated antigen SGA-56M; C1orf16; chromosome 1 open reading frame 16; EST1 telomerase component homolog C; EST1CKIAA0250SGA56M; EST1-like protein C; ever shorter telomeres 1C; FLJ23717; hSMG-7; protein SMG7; SMG-7 homolog; Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans); SMG-7 SMG7 C1orf16, EST1C, SGA56M SMG7 nonsense mediated mRNA decay factor protein SMG7|EST1 telomerase component homolog C|EST1-like protein C|breast cancer-associated antigen SGA-56M|ever shorter telomeres 1C|smg-7 homolog, nonsense mediated mRNA decay factor

For more info on SMG7, check out the SMG7 Infographic

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In this infographic, you will see the following information for SMG7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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