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Product Info Summary
| SKU: | M02357 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Mad2L2 Monoclonal Antibody
SKU/Catalog Number
M02357
BM5428 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Mad2L2 Monoclonal Antibody catalog # M02357. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mad2L2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02357)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADFO-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Mad2L2
Reactive Species
M02357 is reactive to MAD2L2 in Human, Mouse, Rat
Observed Molecular Weight
24 kDa
Calculated molecular weight
24.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02357 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:30
FC 1:40
Positive Control
WB: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of Mad2L2 expression in Hela cell lysate.
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Expression of STAM, ANXA5 and MAD2L2 in HCC cell lines. A The expressions of STAM, ANXA5 and MAD2L2 in normal hepatocytes and hepatoma cell lines were detected using Western blotting. Knocking down STAM, ANXA5 and MAD2L2 inhibited the proliferation and migration of HCC cells. B The knockdown efficiency of STAM, ANXA5 and MAD2L2 was detected using Western Blotting. C Plate cloning experiment showed that the number of cloned cell clusters formed by hepatocellular carcinoma cells after knockdown of STAM, ANXA5 and MAD2L2 was significantly reduced; D The results of CCK8 experiment showed that inhibiting the expression of STAM, ANXA5 and MAD2L2 decreased the proliferative ability of HCC cells; E Scratch assay showed that knockdown of STAM, ANXA5 and MAD2L2 inhibited the migration of hepatocellular carcinoma cells; F Transwell experiment showed that the migration ability of hepatocellular carcinoma cells was weakened after inhibiting the expression of STAM, ANXA5 and MAD2L2 .* P < 0.05, ** P < 0.01, *** P < 0.001, there was no significant difference in ns
Index in PubMed under a CC BY license. PMID: 38049741
Specific Publications For Anti-Mad2L2 Monoclonal Antibody (M02357)
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7 Customer Q&As for Anti-Mad2L2 Monoclonal Antibody
Question
Here is the WB image, lot number and protocol we used for cerebellum embryo using anti-Mad2L2 Monoclonal antibody M02357. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-24
Question
Is a blocking peptide available for product anti-Mad2L2 Monoclonal antibody (M02357)?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
We do provide the blocking peptide for product anti-Mad2L2 Monoclonal antibody (M02357). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-30
Question
Will anti-Mad2L2 Monoclonal antibody M02357 work on goat WB with testis?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
Our lab technicians have not tested anti-Mad2L2 Monoclonal antibody M02357 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Mad2L2 Monoclonal antibody M02357 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-20
Question
I see that the anti-Mad2L2 Monoclonal antibody M02357 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-01-10
Question
What sample was tested in WB and IHC applications with M02357?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of Mad2L2 expression in Hela cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
Is this M02357 anti-Mad2L2 Monoclonal antibody reactive to the isotypes of MAD2L2?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
The immunogen of M02357 anti-Mad2L2 Monoclonal antibody is A synthesized peptide derived from human Mad2L2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-13
Question
We are currently using anti-Mad2L2 Monoclonal antibody M02357 for mouse tissue, and we are satisfied with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The anti-Mad2L2 Monoclonal antibody (M02357) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-09
