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SMIM8 antibody (A17404) performs well in ELISA, IHC and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of SMIM8 using anti-SMIM8 antibody (A17404).
SMIM8 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMIM8 Antibody (A17404) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
SMIM8 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMIM8 Antibody (A17404) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-SMIM8 Antibody
SMIM8 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IHC |
Tested Applications
SMIM8 antibody was positively detected by IHC in:
human colon tissue, human diffuse large B cell lymphoma tissue, human laryngeal squamous cell carcinoma tissue, human liver cancer tissue, human lung cancer tissue, human placenta tissue, human spleen tissue, human spleen tissue, human testicular seminoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
ELISA
Human
Tested
Recommend
Recommend
Rabbit
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-SMIM8 Antibody
View all SMIM8 Antibodies
View all SMIM8 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human SMIM8 recombinant protein (Position: M1-D97). Human SMIM8 shares 88.7% amino acid (aa) sequence identity with mouse SMIM8.
Cross-Reactivity
No cross reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Calculated Molecular Weight
11.1 kDa
Background of SMIM8
This gene is predicted to be integral component of membrane.
Cite This Product
Anti-SMIM8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17404)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
94%
High Homology
Dog
93%
High Homology
Cow
91%
High Homology
Mouse
89%
Moderate Homology
Sheep
89%
Moderate Homology
Rat
87%
Moderate Homology
Zebrafish
64%
Low homology
Drosophila
30%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for SMIM8 antibody
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Product Promise
We promise Anti-SMIM8 Antibody performs excellently in relevant applications for ELISA, IHC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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A17404
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026